Summary of Study ST000605
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000442. The data can be accessed directly via it's Project DOI: 10.21228/M8XC7Q This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST000605 |
| Study Title | Whole blood reveals more metabolic detail of the human metabolome than serum as measured by 1H-NMR spectroscopy: Implications for sepsis metabolomics |
| Study Type | Single timepoint; healthy controls |
| Study Summary | Healthy volunteers |
| Institute | University of Michigan |
| Department | Clinical Pharmacy |
| Laboratory | The NMR Metabolomics Laboratory (Stringer) |
| Last Name | Stringer |
| First Name | Kathleen |
| Address | University of Michigan, 2900 Huron Parkway, Ann Arbor, MI 48105 |
| NMRmetabolomics@umich.edu | |
| Phone | NA |
| Submit Date | 2016-12-08 |
| Total Subjects | 19 whole blood; 20 serum |
| Raw Data Available | Yes |
| Raw Data File Type(s) | fid |
| Analysis Type Detail | NMR |
| Release Date | 2017-01-06 |
| Release Version | 1 |
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Project:
| Project ID: | PR000442 |
| Project DOI: | doi: 10.21228/M8XC7Q |
| Project Title: | Whole blood and serum 1D-1H-NMR metabolomics |
| Project Type: | Cross-center Quantitative NMR Urine Metabolomics Study |
| Project Summary: | Serum is a common sample of convenience for metabolomics studies. Its processing time can be lengthy and may result in the loss of metabolites including those of red blood cells (RBC). Unlike serum, whole blood (WB) can be quickly processed, minimizing the influence of hemolysis without excluding RBC metabolites. To determine differences between serum and WB metabolomes, both sample types, collected from healthy volunteers, were assayed by 1H-NMR spectroscopy. A total of 34 and 51 aqueous metabolites were quantified from serum and WB, respectively. Free hemoglobin (Hgb) levels in serum were measured and the correlation between Hgb and serum metabolites was determined. All metabolites detected in serum were detected at higher concentrations in WB with the exception of acetoacetate and propylene glycol. The 18 unique metabolites of WB included adenosine, AMP, ADP and ATP, which are associated with RBC metabolism. The use of serum results in the underrepresentation of a number of metabolic pathways including branched chain amino acid degradation and glycolysis and gluconeogenesis. The range of free Hgb in serum was 0.03-0.010 g/dL and 8 metabolites were associated (p < 0.05) with free Hgb. The range of free Hgb in serum samples from 18 sepsis patients was 0.02-0.46 g/dL. WB and serum have unique aqueous metabolite profiles but the use of serum may introduce potential pathway bias. Use of WB for metabolomics may be particularly important for studies in diseases like sepsis in which RBC metabolism is altered and mechanical and sepsis-induced hemolysis contributes to variance in the metabolome. This study has been published: Shock 2015;44:200-208. |
| Institute: | University of Michigan;University of Mississippi |
| Department: | Emergency Medicine |
| Laboratory: | NMR Metabolomics Laboratory, University of Michigan |
| Last Name: | Stringer |
| First Name: | Kathleen |
| Address: | College of Pharmacy, University of Michigan, 428 Church Street, Ann Arbor, MI 48109 |
| Email: | NMRmetabolomics@umich.edu |
| Phone: | NA |
| Funding Source: | NIH (GM069438, GM113041); Upjohn Award, College of Pharmacy |
