Summary of Study ST001056
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000709. The data can be accessed directly via it's Project DOI: 10.21228/M81M4X This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST001056 |
| Study Title | Evaluation of the short-term effects of the allelochemical umbelliferone on Triticum durum L. metabolism through GC-MS based untargeted metabolomics |
| Study Summary | Allelopathy is a plant defense mechanism by which they protect themselves from competitive species using specialized biochemicals in the form of secretion or volatiles released to the environment. Though, umbelliferone is a well-known allelochemical, its mechanism of action in a short-term treatment is far from established. We used ≈ 10–days old wheat seedlings treated with104 µM umbelliferone over a time course experiment covering 6 times points, i.e., 0h, 6h, 12h, 24h, 48h, and 96h and compared the metabolomic changes to control (mock-treated) plants. Using gas chromatography mass-spectrometry (GC-MS) based metabolomics efforts, we collectively obtained quantitative data on 177 metabolites that were derivatized (either derivatized singly or multiple times) or not representing 139 non-redundant (unique) metabolites. Out of these 139 metabolites, 118 were associated with a unique HMDB identifier, while 113 were associated with a KEGG identifier. Relative quantification of these metabolites across the time-course of umbelliferone treatment, revealed 22 compounds (sugars, fatty acids, secondary metabolites, organic acids, and amino acids) that changed significantly (repeated measures ANOVA, P-value < 0.05) with time. Using multivariate partial least squares discriminant analysis (PLS-DA) we showed the grouping of samples based on time-course across the control and umbelliferone treated plants, whereas the metabolite-metabolite Pearson correlation revealed tightly formed clusters of umbelliferone-derived metabolites, fatty acids, amino acids, and carbohydrates. Also, time-course of umbelliferone treatment revealed, that phospho-L-serine, maltose, and dehydroquinic acid were the top three metabolites showing highest importance in discrimination among the time-points. The above indicate a system-wide changes induced by umbelliferone, through dysregulation of primary as well as specialized metabolism. |
| Institute | Università Mediterranea di Reggio Calabria |
| Department | Dipartimento AGRARIA |
| Last Name | Araniti |
| First Name | Fabrizio |
| Address | Department AGRARIA, University Mediterranea of Reggio Calabria, Località Feo di Vito, SNC I-89124, Reggio Calabria RC, Italy |
| fabrizio.araniti@unirc.it | |
| Phone | +39-09651694283 |
| Submit Date | 2018-09-12 |
| Num Groups | 3 |
| Publications | In process |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | GC-MS |
| Release Date | 2020-01-13 |
| Release Version | 1 |
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Project:
| Project ID: | PR000709 |
| Project DOI: | doi: 10.21228/M81M4X |
| Project Title: | Short-term effects of the phytotoxic allelochemicals umbelliferone on Triticum durum L. metabolism |
| Project Summary: | Short-term effects of the phytotoxic allelochemicals umbelliferone on Triticum durum L. metabolism |
| Institute: | Università Mediterranea di Reggio Calabria |
| Department: | Dipartimento AGRARIA |
| Last Name: | Misra |
| First Name: | Biswapriya |
| Address: | Medical Center Boulevard NRC Building, G#43, Medical Center Boulevard, Winston Salem, NC, 27157, USA |
| Email: | bbmisraccb@gmail.com |
| Phone: | 3522156040 |
| Funding Source: | NA |
| Project Comments: | NA |
| Publications: | In process |
| Contributors: | Biswapriya B. Misra, Araniti F, Abenavoli MR |
