Summary of Study ST001272

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000856. The data can be accessed directly via it's Project DOI: 10.21228/M81T3M This work is supported by NIH grant, U2C- DK119886.

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Study IDST001272
Study TitleGrowth cone memebrane and growth cone particulate lipidomics
Study Typeuntargeted LC-MS/MS lipidomics
Study SummaryWe performed high performance liquid chromatography tandem mass spectrometry (LC-MS/MS) analysis of the proteome and lipidome of GC from C57BL/6 mice across five age groups (E18, P0, P3, P6, and P9) from two growth cone fractions: growth cone membrane (GCM) and growth cone particulate (GCP)
Institute
University of Miami
DepartmentOphthalmology, Bascom Palmer Eye Institute
LaboratorySanjoy K. Bhattacharya
Last NameBhattacharya
First NameSanjoy
Address1638 NW 10th Avenue, Miami, FL 33136
EmailSBhattacharya@med.miami.edu
Phone305-482-4103
Submit Date2019-10-21
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2020-01-03
Release Version1
Sanjoy Bhattacharya Sanjoy Bhattacharya
https://dx.doi.org/10.21228/M81T3M
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR000856
Project DOI:doi: 10.21228/M81T3M
Project Title:Growth cone Membrane lipidomics and Growth cone particulate at different developmental stages in C57BL/6 mice
Project Type:untargeted LC-MS/MS lipidomics
Project Summary:We performed high-performance liquid chromatography tandem mass spectrometry (LC-MS/MS) analysis of the lipidome of GC from C57BL/6 mice across five age groups (E18, P0, P3, P6, and P9) from two growth cone fractions: growth cone membrane (GCM) and growth cone particulate (GCP). These fractions were generated through established differential centrifugation techniques and lipid extraction. Developmental time points were chosen based upon our understanding of brain development in the murine model. In mice cortex, E18 is characterized by extension or elongation of axons toward targets, while around P0-P3 is when axons start innervating targets and branching occurs. At P6-P7 is when target innervation is generally complete and a subpopulation of cortical neurons undergo developmental cell death. By P9 growth cones have mostly disappeared.
Institute:University of Miami
Department:Ophthalmology, Bascom Palmer Eye Institute
Laboratory:Sanjoy K. Bhattacharya
Last Name:Bhattacharya
First Name:Sanjoy
Address:1638 NW 10th Avenue Miami, FL 33136
Email:SBhattacharya@med.miami.edu
Phone:305-482-4103
Contributors:Jennifer Arcuri
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