Summary of Study ST001451
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000997. The data can be accessed directly via it's Project DOI: 10.21228/M8TH75 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001451 |
| Study Title | Eleostearic acid effects on TAGs and oxLipids |
| Study Summary | Quantification of lipid species from cultured human MDA-MB-231 and BT549 cells with or without siRNA knockdown of ACSL1 and treated or not with eleostearic acid |
| Institute | Fox Chase Cancer Center |
| Last Name | Peterson |
| First Name | Jeffrey |
| Address | 333 Cottman Avenue Philadelphia, PA 19111 |
| Jeffrey.peterson@fccc.edu | |
| Phone | 215-728-3568 |
| Submit Date | 2020-08-20 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2020-09-10 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR000997 |
| Project DOI: | doi: 10.21228/M8TH75 |
| Project Title: | ACSL1 role in conjugated PUFA metabolism |
| Project Type: | MS quantitative lipidomic study |
| Project Summary: | Ferroptosis is associated with lipid hydroperoxides generated by oxidation of polyunsaturated acyl chains. Lipid hydroperoxides are reduced by glutathione peroxidase 4 (GPX4) and GPX4 inhibitors induce ferroptosis. However, the therapeutic potential of triggering ferroptosis in cancer cells with polyunsaturated fatty acids is unknown. We identified conjugated linoleates including α-eleostearate (αESA) as novel ferroptosis inducers. αESA did not alter GPX4 activity but was incorporated into cellular lipids and promoted lipid peroxidation and cell death in diverse cancer cell types. αESA-triggered death was mediated by acyl-CoA synthetase long-chain isoform 1, which promoted αESA incorporation into neutral lipids including triacylglycerols. Interfering with triacylglycerol biosynthesis suppressed ferroptosis triggered by αESA. |
| Institute: | Fox Chase Cancer Center |
| Last Name: | Peterson |
| First Name: | Jeffrey |
| Address: | 333 Cottman Avenue, Philadelphia, PA, 19111, USA |
| Email: | Jeffrey.peterson@fccc.edu |
| Phone: | 215-728-3568 |
| Funding Source: | DOD, NIH |
| Publications: | in process |
| Contributors: | Valarian Kagan |
