Summary of Study ST001687

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001085. The data can be accessed directly via it's Project DOI: 10.21228/M8FT4Q This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001687
Study TitleNon-transformed cells respond to fat by inducing glucose metabolism
Study SummaryC57BL/6N mice were obtained from the KU Leuven animal laboratory. 2-week old mice were injected i.p. diethylnitrosamine (DEN, 25 mg/Kg) in phosphate buffered saline (3.17 mg/ml).s At 6-weeks of age, mice were randomized into two groups: control diet (CD, E15742-33 ssniff Spezialdiäten GmbH) or high fat diet (HFD, S8655-E220 sniff Spezialdiäten GmbH). At endpoint, mice were sacrificed by injecting approximately 50 µl of a 60 mg/ml Nembutal solution (Vetoquinol). Tissues were immediately excised, washed in ice-cold saline, placed into pre-labelled bags and frozen using a liquid nitrogen-cooled biosqueezer. The bags were then placed in liquid nitrogen until all collections were finished and finally stored at -80°C until further processing. Where necessary tumors were rapidly separated from normal tissue prior to freezing with tumor and normal tissue being stored separately.
Institute
VIB-KU Leuven Center for Cancer Biology
DepartmentDepartment of Oncology
LaboratoryLaboratory of Cellular Metabolism and Metabolic Regulation
Last NameFendt
First NameSarah-Maria
AddressON IV Herestraat 49 - box 912 3000 Leuven
Emailsarah-maria.fendt@kuleuven.be
Phone+32 16 37 32 61
Submit Date2021-02-04
Num Groups4
Total Subjects16
Num Males16
Raw Data AvailableYes
Raw Data File Type(s)mzML
Analysis Type DetailLC-MS
Release Date2021-02-27
Release Version1
Sarah-Maria Fendt Sarah-Maria Fendt
https://dx.doi.org/10.21228/M8FT4Q
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR001085
Project DOI:doi: 10.21228/M8FT4Q
Project Title:Non-transformed cells respond to fat by inducing glucose metabolism
Project Summary:In this study, C57BL/6 mice were exposed to control or high fat diet, and PBS control or diethylnitrosamine (DEN) injections for stimulation of hepatocellular carcinoma. This dataset provides untargeted lipidomics in liver tissue from PBS-injected control and high fat diet fed mice, and paired tumor tissue and adjacent liver tissue from DEN-injected, high fat diet fed mice.
Institute:VIB-KU Leuven Center for Cancer Biology
Department:Department of Oncology
Laboratory:Laboratory of Cellular Metabolism and Metabolic Regulation
Last Name:Fendt
First Name:Sarah-Maria
Address:ON IV Herestraat 49 - box 912 3000 Leuven
Email:sarah-maria.fendt@kuleuven.be
Phone:+32 16 37 32 61
Contributors:Sarah-Maria Fendt, Lindsay A. Broadfield, Jonas Dehairs, Johannes V. Swinnen
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