Summary of Study ST001760
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001127. The data can be accessed directly via it's Project DOI: 10.21228/M81D6M This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001760 |
| Study Title | Application of the redox metabolite detection method for mouse kidney |
| Study Summary | This study was aimed at optimizing redox metabolites detection from mammalian tissues. Three different chromatographic conditions were compared as well as three different extraction conditions. This study was run on ZIC-pHILIC chromatography. This study is an independent replicate |
| Institute | Boston Children's Hospital, Harvard Medical School |
| Department | Pathology |
| Laboratory | Naama Kanarek |
| Last Name | Petrova |
| First Name | Boryana |
| Address | 300 Longwood Av, Boston, MA, 2115, USA |
| boryana.petrova@childrens.harvard.edu | |
| Phone | 6173557433 |
| Submit Date | 2021-04-21 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2021-05-17 |
| Release Version | 1 |
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Project:
| Project ID: | PR001127 |
| Project DOI: | doi: 10.21228/M81D6M |
| Project Title: | Redox metabolism measurement in mammalian cells and tissues by quantitative LC/MS method (part I) |
| Project Summary: | This study aimed to optimize the detection of several key redox-reactive metabolites from mammalian cells and tissues. We explored three different chromatographic methods and optimized sample preparation, extraction buffer and conditions as well as mass spectrometry detection parameters. The established method was tested and validated using biologically relevant ROS-inducing conditions. This study can be a valuable resource for the metabolomics community. |
| Institute: | Boston Childrens Hospital |
| Department: | Pathology |
| Laboratory: | Naama Kanarek |
| Last Name: | Petrova |
| First Name: | Boryana |
| Address: | 300 Longwood Av, Boston, MA, 2115, USA |
| Email: | boryana.petrova@childrens.harvard.edu |
| Phone: | 6173557433 |
