Summary of Study ST002412

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001552. The data can be accessed directly via it's Project DOI: 10.21228/M8342Z This work is supported by NIH grant, U2C- DK119886.

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This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST002412
Study TitleMetabolic effects of the protein kinase R
Study TypeBiomedical research
Study SummarySpleen-derived macrophage from WT or Eif2ak2-/- (gene encoding PKR protein kinase) mice are treated with a synthetic RNA mimetic (polyinosinic:polycytidylic acid) to activate the kinase and metabolites were collected for analysis. The data identified 325 putative metabolites in the cell extracts, with a large number of significant differences between the Eif2ak2- /- and WT sample groups. Metabolite levels are predominantly suppressed in the WT compared to the Eif2ak2-/- cells, with depletion of specific metabolites in amino acid, carbohydrate, lipid and nucleotide pathways, while several amino acid metabolites were significantly elevated in the WT cells compared to the Eif2ak2-/-. The changes appear to delineate a pseudo-starvation response in the WT cells. Phosphate energy metabolism is altered with decreased creatine and phosphocreatine and a compensatory increase in phosphorylated guanidinoacetate in the WT compared to the Eif2ak2- /- cells. There appears to be a constraint in glycolysis in the WT cells, most clearly in the pentose phosphate pathway.
Institute
Hudson Institute of Medical Research
DepartmentCIIID
LaboratoryMolecular Immunology
Last NameSadler
First NameAnthony
Address27-31 Wright st, Clayton, VIC 3168
EmailAnthony.sadler@hudson.org.au
Phone+61 4 85722722
Submit Date2022-12-15
Num Groups2
Total SubjectsNA
Num MalesNA
Num FemalesNA
Study CommentsKO vs WT
PublicationsSuppression of the nucleic acid precursor ribose 5-phosphate by RNA-mediated antiviral immunity
Raw Data AvailableYes
Raw Data File Type(s)raw(Thermo)
Analysis Type DetailLC-MS
Release Date2023-01-04
Release Version1
Anthony Sadler Anthony Sadler
https://dx.doi.org/10.21228/M8342Z
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Project:

Project ID:PR001552
Project DOI:doi: 10.21228/M8342Z
Project Title:Metabolic effects of the protein kinase R
Project Type:Untargeted LCMS metabolomics
Project Summary:Spleen-derived macrophage from WT or Eif2ak2-/- (gene encoding PKR protein kinase) mice are treated with a synthetic RNA mimetic (polyinosinic:polycytidylic acid) to activate the kinase and metabolites were collected for analysis. The data identified 325 putative metabolites in the cell extracts, with a large number of significant differences between the Eif2ak2- /- and WT sample groups. Metabolite levels are predominantly suppressed in the WT compared to the Eif2ak2-/- cells, with depletion of specific metabolites in amino acid, carbohydrate, lipid and nucleotide pathways, while several amino acid metabolites were significantly elevated in the WT cells compared to the Eif2ak2-/-. The changes appear to delineate a pseudo-starvation response in the WT cells. Phosphate energy metabolism is altered with decreased creatine and phosphocreatine and a compensatory increase in phosphorylated guanidinoacetate in the WT compared to the Eif2ak2- /- cells. There appears to be a constraint in glycolysis in the WT cells, most clearly in the pentose phosphate pathway.
Institute:Hudson Institute of Medical Research
Department:CIIID
Laboratory:Molecular Immunology
Last Name:Sadler
First Name:Anthony
Address:27-31 Wright St, Clayton, VIC 3168, Australia
Email:Anthony.sadler@hudson.org.au
Phone:+61 4 85722722
Funding Source:NHMRC grants (1143839, 1043398) and a philanthropic Perpetual Trusties grant (CF07/2408) and a philanthropic Perpetual IMPACT grant.
Publications:Suppression of the nucleic acid precursor ribose 5-phosphate by RNA-mediated antiviral immunity
Contributors:Pushpack Bhattacharjee, Die Wang, Dovile Anderson, Joshua N. Buckler, Eveline de Geus, Feng Alex Yan, Galina Polekhina, Ralf Schittenhelm, Darren J. Creek, Lawrence D. Harris, Anthony J. Sadler
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