Summary of Study ST002828
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR001770. The data can be accessed directly via it's Project DOI: 10.21228/M8XB0Q This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST002828 |
Study Title | Role of the Afferent Lymph as an Immunological Conduit to Analyze Tissue Antigenic and Inflammatory Load - metabolomics |
Study Summary | The intestinal barrier, and the gut-associated lymphoid tissue, are the interface of the host-microbiome/pathogens interactions and, its disruption, has been associated with a series of inflammatory, autoimmune, and degenerative diseases. Herein we performed the analysis of the pre-nodal mesenteric lymph at steady state and following disruption of the intestinal barrier to map the antigenic and pro-inflammatory load in both mice and human subjects with inflammatory bowel disease. The antigenic signature of gastrointestinal tissue inflammation was reflected in the mesenteric nodal dendritic-cells (DC)-MHCII-eluted immunopeptidome, in a tissue specific manner, when compared to the DC-eluted-MHCII-peptidome from cervical lymph nodes. Pro-inflammatory and microbiome-derived by-products, such as amino acids, deoxy sugars, component of the bacterial wall and other immunomodulators of the gut-brain axis, were found in the afferent lymph following damage to the gut epithelium. Our data points to the relevance of the lymphatic fluid to probe the tissue-specific antigenic and inflammatory load transported to the draining lymph node. |
Institute | University of Colorado Denver |
Last Name | Haines |
First Name | Julie |
Address | 12801 E 17th Ave, Room 1303, Aurora, Colorado, 80045, USA |
julie.haines@cuanschutz.edu | |
Phone | 3037243339 |
Submit Date | 2023-08-21 |
Raw Data Available | Yes |
Raw Data File Type(s) | raw(Thermo) |
Analysis Type Detail | LC-MS |
Release Date | 2024-08-21 |
Release Version | 1 |
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Project:
Project ID: | PR001770 |
Project DOI: | doi: 10.21228/M8XB0Q |
Project Title: | Role of the Afferent Lymph as an Immunological Conduit to Analyze Tissue Antigenic and Inflammatory Load - metabolomics |
Project Summary: | The intestinal barrier, and the gut-associated lymphoid tissue, are the interface of the host-microbiome/pathogens interactions and, its disruption, has been associated with a series of inflammatory, autoimmune, and degenerative diseases. Herein we performed the analysis of the pre-nodal mesenteric lymph at steady state and following disruption of the intestinal barrier to map the antigenic and pro-inflammatory load in both mice and human subjects with inflammatory bowel disease. The antigenic signature of gastrointestinal tissue inflammation was reflected in the mesenteric nodal dendritic-cells (DC)-MHCII-eluted immunopeptidome, in a tissue specific manner, when compared to the DC-eluted-MHCII-peptidome from cervical lymph nodes. Pro-inflammatory and microbiome-derived by-products, such as amino acids, deoxy sugars, component of the bacterial wall and other immunomodulators of the gut-brain axis, were found in the afferent lymph following damage to the gut epithelium. Our data points to the relevance of the lymphatic fluid to probe the tissue-specific antigenic and inflammatory load transported to the draining lymph node. |
Institute: | University of Colorado Denver |
Laboratory: | Lab of Angelo D'Alessandro in collaboration with lab of Laura Santambrogio (Weill Cornell) |
Last Name: | Haines |
First Name: | Julie |
Address: | 12801 E 17th Ave, Room 1303, Aurora, Colorado, 80045, USA |
Email: | julie.haines@cuanschutz.edu |
Phone: | 3037243339 |