Summary of Study ST003246
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002016. The data can be accessed directly via it's Project DOI: 10.21228/M81C00 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003246 |
| Study Title | Effects of mitoregulin loss on cardiac and mitochondrial lipids in aged male mice |
| Study Summary | Cardiac lipidome analysis in aged (21 to 23-months old) male wildtype and mtln knockout mice |
| Institute | University of Iowa |
| Last Name | Boudreau |
| First Name | Ryan |
| Address | 4334 PBDB, 169 Newton Rd, Iowa City, IA 52242 |
| ryan-boudreau@uiowa.edu | |
| Phone | 3193535573 |
| Submit Date | 2024-06-02 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | mzML |
| Analysis Type Detail | LC-MS |
| Release Date | 2024-12-31 |
| Release Version | 1 |
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Project:
| Project ID: | PR002016 |
| Project DOI: | doi: 10.21228/M81C00 |
| Project Title: | Effects of mitoregulin loss on cardiac and mitochondrial lipids in mice |
| Project Summary: | We and others discovered a highly-conserved mitochondrial transmembrane microprotein, named Mitoregulin (Mtln), that supports lipid metabolism. We reported that Mtln strongly binds cardiolipin (CL), increases mitochondrial respiration and Ca2+ retention capacities, and reduces reactive oxygen species (ROS). Here we extend our observation of Mtln-CL binding and examine Mtln influence on cristae structure and mitochondrial membrane integrity during stress. We demonstrate that mitochondria from constitutive- and inducible Mtln-knockout (KO) mice are susceptible to membrane freeze-damage and that this can be rescued by acute Mtln re-expression. In mitochondrial-simulated lipid monolayers, we show that synthetic Mtln decreases lipid packing and monolayer elasticity. Lipidomics revealed that Mtln-KO heart tissues show broad decreases in 22:6-containing lipids and increased cardiolipin damage/remodeling. Lastly, we demonstrate that Mtln-KO mice suffer worse myocardial ischemia-reperfusion injury, hinting at a translationally-relevant role for Mtln in cardioprotection. Our work supports a model in which Mtln binds cardiolipin and stabilizes mitochondrial membranes to broadly influence diverse mitochondrial functions, including lipid metabolism, while also protecting against stress. |
| Institute: | University of Iowa |
| Last Name: | Boudreau |
| First Name: | Ryan |
| Address: | 4334 PBDB, 169 Newton Rd, Iowa City, IA 52242 |
| Email: | ryan-boudreau@uiowa.edu |
| Phone: | 3193535573 |
