Summary of Study ST003643
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR002253. The data can be accessed directly via it's Project DOI: 10.21228/M8DC25 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST003643 |
| Study Title | Metabolite analysis for WT and BnaMYB52 mutants by LC-MS/MS |
| Study Summary | BnaA09.MYB52 directly targets the BnaBAN promoters and promotes BnaBAN expression in Brassica napus. BAN, encoding anthocyanidin reductase that converts anthocyanidins to 2,3- cis-flavan-3-ols compounds (proanthocyanidins starter units), is involved in the flavonoid biosynthesis pathway. Thus, Metabolite analysis was conducted to detect the content of flavonoid in WT (Wild-type), OE (BnaA09.MYB52 overexpression lines in the genetic background Westar) and mutants (four homologous genes of BnaMYB52 knocked out) plants. About 0.1 g mature seeds were collected from WT, OE and mutant plants. Metabolites analysis demonstrated that BnaMYB52 positively regulated the content of several metabolites (such as L-phenylalanine, p-coumaric acid, grosvenorine and astragalin) in flavonoid pathway. |
| Institute | Huazhong Agricultural University |
| Last Name | Jiang |
| First Name | Ye |
| Address | Shi Zishan Street 1th, Wuhan, Hubei, 430070, China |
| vyejiang@163.com | |
| Phone | 13697353446 |
| Submit Date | 2024-12-21 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | wiff |
| Analysis Type Detail | LC-MS |
| Release Date | 2025-01-26 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Project:
| Project ID: | PR002253 |
| Project DOI: | doi: 10.21228/M8DC25 |
| Project Title: | Metabolite analysis for WT and BnaMYB52 mutants by LC-MS/MS |
| Project Type: | LC-MS/MS |
| Project Summary: | BnaA09.MYB52 directly targets the BnaBAN promoters and promotes BnaBAN expression in B. napus. BAN, encoding anthocyanidin reductase that converts anthocyanidins to 2,3- cis-flavan-3-ols compounds (proanthocyanidins starter units), is involved in the flavonoid biosynthesis pathway. Thus, Metabolite analysis was conducted to detect the content of flavonoid in WT (Wild-type), OE (BnaA09.MYB52 overexpression lines in the genetic background Westar) and mutants (four homologous genes of BnaMYB52 knocked out) plants. The result showed that the content of several metabolites in flavonoid pathway was reduced in mutants, whereas overexpression lines resulted in the opposite trend compared with WT. |
| Institute: | Huazhong Agricultural University |
| Last Name: | Jiang |
| First Name: | Ye |
| Address: | Shi Zishan Street 1th, Wuhan, Hubei, 430070, China |
| Email: | vyejiang@163.com |
| Phone: | 13697353446 |
| Publications: | Ye et al. Manipulation of seed coat content for increasing oil content via modulating BnaMYB52 in Brassica napus. Cell reports |
