Summary of Study ST000014
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000013. The data can be accessed directly via it's Project DOI: 10.21228/M8G59C This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000014 |
| Study Title | Skeletal Muscle Treatment (palmitate/palmitoyl carnitine) |
| Study Type | MS analysis |
| Study Summary | Isolated skelelal muscle mitochondria incubated in the presence of fatty acid oxidation precursors, palmitic acid and palmitoyl carnitine |
| Institute | University of California, Davis |
| Department | Davis Genome Center |
| Laboratory | Fiehn |
| Last Name | Kind |
| First Name | Tobias |
| Address | 451 E. Health Sci. Drive, Davis, California 95616, USA |
| tkind@ucdavis.edu | |
| Submit Date | 2013-05-20 |
| Num Groups | 12 |
| Total Subjects | 72 |
| Raw Data Available | No |
| Analysis Type Detail | GC-MS |
| Release Date | 2013-06-19 |
| Release Version | 1 |
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Sample Preparation:
| Sampleprep ID: | SP000027 |
| Sampleprep Summary: | - |
| Sampleprep Protocol ID: | Mitochondrial Matrix Preparation |
| Extraction Method: | Extramitochondrial Buffer Preparation: The Harper lab first entrifuged mitochondrial preparations in order to get the extramitochondrial buffer and mitochondrial pellet. Mitochondrial Matrix Preparation: the mitochondrial matrix was exposed by repeated freeze-thaws of the mitochondrial pellet; the insoluble material was spun down, and supernatant collected as matrix. Pellets were resuspended in 100 ul of buffer (140 mM KCL, 20 HEPES, 5 K2HPO4, 1 EGTA, pH 7.4 at RT) |
| Organ: | skeletal muscle |
| Organ Specification: | total |
| Cell Type: | myocyte |
| Subcellular Location: | extramitochondrial buffer |
