Summary of Study ST000015

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000013. The data can be accessed directly via it's Project DOI: 10.21228/M8G59C This work is supported by NIH grant, U2C- DK119886.

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Study IDST000015
Study TitleSkeletal Muscle Treatment (palmitate and inhibitors)
Study TypeMS analysis
Study SummaryIsolated skelelal muscle mitochondria incubated in the presence of fatty acid oxidation precursor and inhibitors
Institute
University of California, Davis
DepartmentDavis Genome Center
LaboratoryFiehn
Last NameKind
First NameTobias
Address451 E. Health Sci. Drive, Davis, California 95616, USA
Emailtkind@ucdavis.edu
Submit Date2013-05-20
Num Groups20
Total Subjects125
Raw Data AvailableNo
Analysis Type DetailGC-MS
Release Date2013-06-19
Release Version1
Tobias Kind Tobias Kind
https://dx.doi.org/10.21228/M8G59C
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Sample Preparation:

Sampleprep ID:SP000028
Sampleprep Summary:-
Sampleprep Protocol ID:Extramitochondrial Buffer Preparation
Extraction Method:The Harper lab first entrifuged mitochondrial preparations in order to get the extramitochondrial buffer and mitochondrial pellet. Mitochondrial Matrix Preparation: the mitochondrial matrix was exposed by repeated freeze-thaws of the mitochondrial pellet; the insoluble material was spun down, and supernatant collected as matrix.
Organ:skeletal muscle
Organ Specification:total
Cell Type:myocyte
Subcellular Location:extramitochondrial buffer
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