Summary of study ST000107

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000046. The data can be accessed directly via it's Project DOI: 10.21228/M84S36 This work is supported by NIH grant, U2C- DK119886.


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Study IDST000107
Study TitleMetabolomic and lipidomic profiles in response to exogenous insulin and GLP-1 infusions during prolonged fasting
Study TypeTimecourse
Study SummarySeventeen northern elephant seal pups constituting four different cohorts at Año Nuevo State Reserve were studied at two post-weaning periods: early (1-2 wk post weaning; n=5) and late (6-7 weeks post weaning; n=12). Study #1: Prior to each infusion protocol, plasma U/kg). Following the infusion, blood samples were collected at 10,To determine the effects of prolonged fasting on peripheral insulin activity and function, samples were collected. Ten fasting seal pups (n=5 early, n=5 late) were infused (i.v.) with a mass-specific dose of insulin (0.065 U/kg). Following the infusion, blood samples were collected at 10, 30, 60, 90, and 120 minutes. Study #2: Seven, late-fasted seal pups were administered either a low (LDG; 10 pmol/kg; n=3) or high (HDG; 100 pmol/kg; n=4) dose of GLP-1 immediately following a glucose bolus (0.5 g/kg) (i.v.) infused within 2 mins. the infusions, blood samples were collected at 10, 30, 60, 90, 120, and 150 minutes.
University of California, Davis
Last NameNewman
First NameJohn
Address430 W. Health Sciences Dr., Davis, CA 95616
Submit Date2014-07-10
Num Groups4
Total Subjects5
Raw Data AvailableYes
Raw Data File Type(s)mzML, .MS, .txt, INI
Uploaded File Size751 M
Analysis Type DetailGC/LC-MS
Release Date2015-02-03
Release Version1
John Newman John Newman application/zip

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Sample Preparation:

Sampleprep ID:SP000123
Sampleprep Protocol Filename:Newman_Lab-Endocannabinoid_Extraction_Analysis_Protocol-Ortiz_WCMC.pdf
Processing Storage Conditions:- 20 °C
Extraction Method:SPE
Extract Concentration Dilution:250 µL
Extract Cleanup:SPE
Extract Storage:- 20 °C
Sample Resuspension:100 µL
Sample Spiking:See sample prep protocol file