Summary of Study ST000113

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000102. The data can be accessed directly via it's Project DOI: 10.21228/M88C7T This work is supported by NIH grant, U2C- DK119886.

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Study IDST000113
Study TitleSIRM Analysis of human P493 cells under hypoxia in [U-13C/15N] labeled Glutamine medium (Positive ion mode FTMS)
Study Typetracer
Study SummarySIRM Analysis of MYC inducible P493 cells under normoxia and hypoxia in [U-13C/15N] labeled Glutamine medium
Institute
University of Kentucky
DepartmentToxicology
Last NameFan
First NameTeresa
Emailtwmfan@gmail.com
Submit Date2014-10-08
Num Groups4
Total Subjects8
Study CommentsFor protocols and additional information, see Le, A., Lane, A.N., Hamaker, M., Bose, S., Barbi, J., Tsukamoto, T., Rojas, C.J., Slusher, B.S., Zhang, H., Zimmerman. L.J., Liebler, D.C., Slebos, R.J.C., Lorkiewicz, P.K., Higashi, R.M., Fan, T.W-M., and Dang, C.V. Cell Metabolism 15, 110 (2012). 13C/15N Gln was used as the tracer in this study, even though the analysis did not explicity look for 15N isotopologues.
Raw Data AvailableNo
Analysis Type DetailGC-MS/LC-MS
Release Date2015-02-03
Release Version1
Teresa Fan Teresa Fan
https://dx.doi.org/10.21228/M88C7T
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Sample Preparation:

Sampleprep ID:SP000129
Sampleprep Summary:-
Sampleprep Protocol Comments:Protein extraction and quantification. | Lipid extraction and preparation for FTMS. | Preparation of polar samples for GCMS and NMR.
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