Summary of Study ST000116
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000105. The data can be accessed directly via it's Project DOI: 10.21228/M8W300 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000116 |
Study Title | Comparative metabolomics analysis of the key metabolic nodes in propionic acid synthesis in Propionibacterium acidipropionici |
Study Type | strains comparison |
Study Summary | The parental P. acidipropionici and its genome-shuffled mutant were compared via metabolomics to find the key metabolic nodes influencing PA production. |
Institute | Jiangnan University |
Department | Key Laboratory of Carbohydrate Chemistry and Biotechnology |
Last Name | Guan |
First Name | Ningzi |
Address | 1800 Lihu Ave, Binhu, Wuxi, Jiangsu, China |
caimo-zi@163.com | |
Submit Date | 2014-11-14 |
Raw Data Available | No |
Raw Data File Type(s) | d |
Analysis Type Detail | LC-MS |
Release Date | 2015-02-03 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP000132 |
Sampleprep Summary: | After quenching at 40°C, the cells were pelleted in a centrifuge (4,000 × g, ?4°C, 10 min). The pellets were washed with methanol solution and resuspended in 1 mL 35% perchloric acid to extract the metabolites from the cells. The mixture was frozen in liquid nitrogen and thawed three times. The supernatant was neutralized by adding K2CO3 solution with an initial concentration of 5 M, and an extract containing all metabolites was collected via centrifugation at 10,000 × g for 5 min at 4°C |
Processing Method: | Homogenization and solvent removal |
Processing Storage Conditions: | on ice |
Extraction Method: | 35% perchloric acid and 5 M K2CO3 |
Extract Storage: | -80°C |
Cell Type: | wildtype cells, genome shffled cells |