Summary of study ST000116

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000105. The data can be accessed directly via it's Project DOI: 10.21228/M8W300 This work is supported by NIH grant, U2C- DK119886.


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Study IDST000116
Study TitleComparative metabolomics analysis of the key metabolic nodes in propionic acid synthesis in Propionibacterium acidipropionici
Study Typestrains comparison
Study SummaryThe parental P. acidipropionici and its genome-shuffled mutant were compared via metabolomics to find the key metabolic nodes influencing PA production.
Jiangnan University
DepartmentKey Laboratory of Carbohydrate Chemistry and Biotechnology
Last NameGuan
First NameNingzi
Address1800 Lihu Ave, Binhu, Wuxi, Jiangsu, China
Submit Date2014-11-14
Raw Data AvailableNo
Analysis Type DetailLC-MS
Release Date2015-02-03
Release Version1
Ningzi Guan Ningzi Guan application/zip

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Sample Preparation:

Sampleprep ID:SP000132
Sampleprep Summary:After quenching at –40°C, the cells were pelleted in a centrifuge (4,000 × g, ?4°C, 10 min). The pellets were washed with methanol solution and resuspended in 1 mL 35% perchloric acid to extract the metabolites from the cells. The mixture was frozen in liquid nitrogen and thawed three times. The supernatant was neutralized by adding K2CO3 solution with an initial concentration of 5 M, and an extract containing all metabolites was collected via centrifugation at 10,000 × g for 5 min at –4°C
Processing Method:Homogenization and solvent removal
Processing Storage Conditions:on ice
Extraction Method:35% perchloric acid and 5 M K2CO3
Extract Storage:-80°C
Cell Type:wildtype cells, genome shffled cells