Summary of Study ST000119
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000106. The data can be accessed directly via it's Project DOI: 10.21228/M8RC75 This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000119 |
| Study Title | Metabolomics analysis of multiple metabolic functions in the YjgF/YER057c/UK114 (Rid) protein family LCMS |
| Study Type | wildtype vs knock-out |
| Study Summary | Metabolomics analysis of wild-type S. enterica, single RidA knock-out and triple Rid (ridA Rid2 Rid7) knock-out S.enterica cells |
| Institute | University of California, Davis |
| Department | Genome and Biomedical Sciences Facility |
| Laboratory | Fiehn Laboratory |
| Last Name | ElBadawi-Sidhu |
| First Name | Mona |
| Address | 451 Health Sciences Drive, Davis, California 95616, USA |
| mmelbadawi@ucdavis.edu | |
| Submit Date | 2014-11-06 |
| Num Groups | 4 |
| Total Subjects | 6 |
| Study Comments | Samples were measured in poitive and negative ionization mode. MS/MS files contain product ion scans (MS/MS) spectra for compound identification. |
| Raw Data Available | No |
| Raw Data File Type(s) | d |
| Analysis Type Detail | LC-MS |
| Release Date | 2014-11-06 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
| Sampleprep ID: | SP000135 |
| Sampleprep Summary: | - |
| Processing Storage Conditions: | on ice |
| Extraction Method: | 1.1 mL of cold, degassed extraction solvent (3:1 Methanol:Water) was added to each sample. Samples were vortexed for 20 sec then placed in sonicator for 5 min, followed by another 20 sec vortex. Samples were placed in -20C for 30 minutes prior to spinning down in centrifuge at 14 rcf for 5 min. The supernatant was transferred to 1.5 mL eppendorf tubes and evaporated in the Labconco speed vac. |
| Extract Storage: | -80C |
| Sample Resuspension: | H20 + 5mM NH4OAc + 0.2% Acetic Acid |
