Summary of study ST000119

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000106. The data can be accessed directly via it's Project DOI: 10.21228/M8RC75 This work is supported by NIH grant, U2C- DK119886.

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Study IDST000119
Study TitleMetabolomics analysis of multiple metabolic functions in the YjgF/YER057c/UK114 (Rid) protein family LCMS
Study Typewildtype vs knock-out
Study SummaryMetabolomics analysis of wild-type S. enterica, single RidA knock-out and triple Rid (ridA Rid2 Rid7) knock-out S.enterica cells
Institute
University of California, Davis
DepartmentGenome and Biomedical Sciences Facility
LaboratoryFiehn Laboratory
Last NameElBadawi-Sidhu
First NameMona
Address451 Health Sciences Drive, Davis, California 95616, USA
Emailmmelbadawi@ucdavis.edu
Submit Date2014-11-06
Num Groups4
Total Subjects6
Study CommentsSamples were measured in poitive and negative ionization mode. MS/MS files contain product ion scans (MS/MS) spectra for compound identification.
Raw Data AvailableNo
Analysis Type DetailLC-MS
Release Date2014-11-06
Release Version1
Mona ElBadawi-Sidhu Mona ElBadawi-Sidhu
https://dx.doi.org/10.21228/M8RC75
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Sample Preparation:

Sampleprep ID:SP000135
Processing Storage Conditions:on ice
Extraction Method:1.1 mL of cold, degassed extraction solvent (3:1 Methanol:Water) was added to each sample. Samples were vortexed for 20 sec then placed in sonicator for 5 min, followed by another 20 sec vortex. Samples were placed in -20C for 30 minutes prior to spinning down in centrifuge at 14 rcf for 5 min. The supernatant was transferred to 1.5 mL eppendorf tubes and evaporated in the Labconco speed vac.
Extract Storage:-80C
Sample Resuspension:H20 + 5mM NH4OAc + 0.2% Acetic Acid
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