Summary of Study ST000133

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000118. The data can be accessed directly via it's Project DOI: 10.21228/M8B594 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

Perform statistical analysis  |  Show all samples  |  Show named metabolites  |  Download named metabolite data  
Download mwTab file (text)   |  Download mwTab file(JSON)   |  Download data files (Contains raw data)
Study IDST000133
Study Title1H NMR Metabolomics Study of Metastatic Melanoma in C57BL/6J Mouse Spleen
Study TypeTissue Extracts Comparison
Study SummaryTissue extracts from metastatic melanoma mouse spleen and controls were compared via NMR based metabolomic analysis
Institute
Pacific Northwest National Laboratory
DepartmentFundamental & Computational Sciences
Last NameHu
First NameJianzhi
Emailjianzhi.hu@pnnl.gov
Submit Date2015-01-08
Num Groups1
Total Subjects12
Raw Data AvailableYes
Uploaded File Size140 K
Analysis Type DetailNMR
Release Date2015-01-08
Release Version1
Jianzhi Hu Jianzhi Hu
https://dx.doi.org/10.21228/M8B594
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

Select appropriate tab below to view additional metadata details:


Sample Preparation:

Sampleprep ID:SP000149
Sampleprep Summary:Cold Methanol/H2O/Chloroform Extraction
Extraction Method:Step 1: The pre-weighted intact frozen tissue was homogenized in ice bath after adding 0.25 ml MeOH and 0.053 ml H2O for each sample containing 30 to 60 mg of tissue, followed by vortexing the mixture and then adding 0.125 ml chloroform, vortexing again. Step 2: 0.125 ml chloroform and 0.125 ml water was added to the sample and then vortexed again, followed by transferring different layers into glass vials separately with syringes after the mixture being left on ice for 15 min and centrifuged at 8000rpm for 2 min at 4°C. Finally, the solvents were removed by employing lyophilizer (MeOH/H2O layer, hydrophilic metabolites) or by evaporating under dry nitrogen gas (CHCl3 layer, hydrophobic metabolites). The extracts were then stored at -80°C before performing NMR measurements.
  logo