Summary of Study ST000149

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000130. The data can be accessed directly via it's Project DOI: 10.21228/M81P4M This work is supported by NIH grant, U2C- DK119886.


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Study IDST000149
Study TitleHigh Insulin Combined With Essential Amino Acids Stimulates Skeletal Muscle Mitochondrial Protein Synthesis While Decreasing Insulin Sensitivity in Healthy Humans
Study TypeHigh and low insulin with and without essential amino acids
Study SummaryThirty participants were randomized to 3 groups of 10 each with each participant studied twice. Study groups comprised (1) low and high insulin, (2) low insulin with and without EAAs, and (3) high insulin with and without EAAs.
Mayo Clinic
Last NameNair
First NameSreekumaran
Submit Date2015-03-05
Num Groups3
Raw Data AvailableNo
Analysis Type DetailLC-MS
Release Date2015-03-05
Release Version1
Sreekumaran Nair Sreekumaran Nair application/zip

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Sample Preparation:

Sampleprep ID:SP000168
Sampleprep Summary:MMP and muscle fractions were isolated from frozen samples using differential centrifugation (18, 19). Biopsy samples were homogenized with protease and phosphatase inhibitors (Halt; Thermo Fisher Scientific) and centrifuged to pellet myofibrillar (MYO) proteins. The supernatant was centrifuged to pellet mitochondrial (MITO) proteins, and the final supernatant was deproteinated with cold ethanol (1:9, v/v) and then centrifuged to pellet sarcoplasmic (SARC) proteins. Aliquots from MMP, MYO, SARC, and MITO were acid hydrolyzed, and free amino acids were purified using cation exchange columns and then were dried.
Plasma phenylalanine enrichment was determined using gas chromatography (GC) and mass spectrometry (MS) as described previously (19). Samples were derivatized to a heptafluorobutyryl isobutyl ester and identified with a Micromass Quattro Micro triple quadrupole GC-MS system (Waters) operating under negative ion chemical ionization using isobutane as the reactant gas. Selected ion monitoring of m/z 399.2 and 403.2 M + 2 and M + 6 fragments of phenylalanine and the l-[ring-13C6]phenylalanine, respectively, was performed.