Summary of Study ST000221

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000181. The data can be accessed directly via it's Project DOI: 10.21228/M8R30R This work is supported by NIH grant, U2C- DK119886.

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Study IDST000221
Study TitleNormal plasma cells,Low proliferation multiple myeloma and High proliferation multiple myeloma cells
Study Typedifferential metabolomics
Study SummaryCD138 sorted bone marrow plasma cell were obtained from a normal patient, a multiple myelow with slow proliferation and a multiple myelow with rapid proliferation.
Institute
Mayo Clinic
DepartmentHematology
Last NameGonsalves
First NameWilliam
EmailDasari.Surendra@mayo.edu
Submit Date2015-06-20
Num Groups1
Total Subjects1
Raw Data AvailableYes
Raw Data File Type(s)d
Uploaded File Size73 G
Analysis Type DetailLC-MS
Release Date2016-06-18
Release Version1
William Gonsalves William Gonsalves
https://dx.doi.org/10.21228/M8R30R
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Sample Preparation:

Sampleprep ID:SP000242
Sampleprep Summary:1) Incubated cell pellets on ice for 15 min
2) Added PBS to cells and vortex well
3) Sonicate for 1 min in ice bath
4) Added MeOH+ACN mixture to cells and vortex well
5) Sonicated for 1 min in ice bath
6) Incubated on ice for 30 min + spin for 20 min at 18K RPM
7) Combined 110 ul from each sample into a pool sample (330 ul total)
8) Split pool into two fractions of 150 ul each (hilic & C18)
9) Split remaning sample into 150 ul x 2 (hilic & C18)
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