Summary of Study ST000236
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000189. The data can be accessed directly via it's Project DOI: 10.21228/M8TS3D This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000236 |
| Study Title | Quick Comparison of Urine Metabolites in Human and SD Rats of Different Sex by Untargeted UPLC-TOFMS and In-house Software Platform |
| Study Type | Biomarker Discovery |
| Study Summary | Human urine samples were collected before breakfast from 14 male and 13 female Chinese post-graduate students, age from 23 to 29, on the morning of sample collection day. Male (n=8) and female (n=8) SD rats weighing between 220 and 250g were used. On the morning of sample collection day, each rat was deprived of food and put in metabolic cage for 24h urine collection. All urine samples were frozen at -80°C prior to analysis. |
| Institute | Beijing Institute of Radiation Medicine |
| Department | Department of Pharmacology and Toxicology |
| Last Name | Liang |
| First Name | Qiande |
| Address | 27 Taiping Road, Beijing, P.R.China |
| liangqiande@yeah.net | |
| Submit Date | 2015-08-09 |
| Num Groups | 4 |
| Total Subjects | 43 |
| Raw Data File Type(s) | raw(Waters) |
| Analysis Type Detail | LC-MS |
| Release Date | 2015-09-09 |
| Release Version | 1 |
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Sample Preparation:
| Sampleprep ID: | SP000260 |
| Sampleprep Summary: | Amygdalin was dissolved in acetonitrile-water(1:1) to form 0.1mg/ml solution. Urine samples were unfrozen at room temperature. 600?l of urine sample was mixed with 20?l Amygdalin solution. After centrifugation at about 14000rpm and -4?, the supernatant was injected into UPLC-TOF MS system. |
| Sampleprep Protocol Filename: | 2_PRE_PROT.pdf |
| Processing Method: | Centrifugation |
| Processing Storage Conditions: | Centrifugation at -4? |
| Extraction Method: | Centrifugation |
| Extract Concentration Dilution: | 600?l of urine/620?l |
| Extract Storage: | 4? after centrifugation and during analysis |
| Sample Spiking: | 20?l Amygdalin solution/620?l |
