Summary of Study ST000237
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000190. The data can be accessed directly via it's Project DOI: 10.21228/M8Q30F This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000237 |
| Study Title | Quick Comparison of Serum Metabolites in SD Rats of Different Sex by Untargeted UPLC-TOFMS and In-house Software Platform |
| Study Type | Biomarker Discovery |
| Study Summary | Male (n=8) and female (n=8) SD rats weighing between 220 and 250g were used. On the morning of sample collection day, each rat was deprived of food and put in metabolic cage for 24h urine collection. Then a blood sample (3-5ml) was collected from the aorta of the rat under anesthesia and centrifuged to obtain serum. All urine and serum samples were frozen at -80°C prior to analysis. |
| Institute | Beijing Institute of Radiation Medicine |
| Department | Department of Pharmacology and Toxicology |
| Last Name | Liang |
| First Name | Qiande |
| Address | 27 Taiping Road, Beijing, P.R.China |
| liangqiande@yeah.net | |
| Submit Date | 2015-08-09 |
| Num Groups | 2 |
| Total Subjects | 16 |
| Raw Data File Type(s) | raw(Waters) |
| Analysis Type Detail | LC-MS |
| Release Date | 2015-09-09 |
| Release Version | 1 |
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Sample Preparation:
| Sampleprep ID: | SP000261 |
| Sampleprep Summary: | Amygdalin was dissolved in acetonitrile-water(1:1) to form 0.1mg/ml solution. Serum samples were unfrozen at room temperature. 500?l of serum sample was mixed with 20?l Amygdalin solution and 100?l 20% Trichloroacetic acid(TCA) solution. After centrifugation at about 14000rpm and -4?, the supernatant was injected into UPLC-TOF MS system. |
| Sampleprep Protocol Filename: | 2_PRE_PROT.pdf |
| Processing Method: | Precipitation |
| Processing Storage Conditions: | Precipitation at room tempreture; centrifugation at -4? |
| Extraction Method: | Precipitation by TCA |
| Extract Concentration Dilution: | 500?l of serum/620?l |
| Extract Storage: | 4? after centrifugation and during analysis |
| Sample Spiking: | 20?l Amygdalin solution/620?l |
