Summary of Study ST000242

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000195. The data can be accessed directly via it's Project DOI: 10.21228/M8630S This work is supported by NIH grant, U2C- DK119886.

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Study ID	ST000242
Study Title	Whole unconditioned medium (Defined culture media, M199),Whole M1 medium,Whole M2 medium
Study Type	differential metabolomics
Study Summary	Media was flash frozen with N2 and stored at -80 C. Samples can be stored at -80 C until use. ~1 ml aliquots. The following media has been provided to the metabolomics core in biological triplicates. Complete (Whole) Media: 1) Whole unconditioned medium (Defined culture media, M199) 2) Whole M1 medium 3) Whole M2 medium
Institute	Mayo Clinic
Department	Physiology and Biomedical Engineering
Last Name	Farrugia
First Name	Gianrico
Email	Dasari.Surendra@mayo.edu
Submit Date	2015-06-26
Num Groups	1
Raw Data Available	Yes
Raw Data File Type(s)	d
Analysis Type Detail	LC-MS
Release Date	2016-07-08
Release Version	2

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Sample Preparation:

Sampleprep ID:	SP000267
Sampleprep Summary:	Quality-control samples used in the study were prepared from pooled samples spiked with a selection of standards. All samples (200 μL) were thawed on ice at 4°C followed by deproteinization with methanol (1:4 ratio of plasma to methanol) and vortexed for 10 s, followed by incubation at −20°C for 2 h. The samples were then centrifuged at 15,871g for 30 min at 4°C. The supernatants were lyophilized (Savant, Holbrook, NY) and stored at −20°C prior to analysis. The samples were reconstituted in 50% H2O/acetonitrile and passed through a Microcon YM3 filter (Millipore Corporation). The supernatants were transferred to analytical vials, stored in the autosampler at 4°C, and analyzed within 48 h of reconstitution in buffer.

Sampleprep ID:

SP000267

Sampleprep Summary:

Quality-control samples used in the study were prepared from pooled samples spiked with a selection of standards. All samples (200 μL) were thawed on ice at 4°C followed by deproteinization with methanol (1:4 ratio of plasma to methanol) and vortexed for 10 s, followed by incubation at −20°C for 2 h. The samples were then centrifuged at 15,871g for 30 min at 4°C. The supernatants were lyophilized (Savant, Holbrook, NY) and stored at −20°C prior to analysis. The samples were reconstituted in 50% H2O/acetonitrile and passed through a Microcon YM3 filter (Millipore Corporation). The supernatants were transferred to analytical vials, stored in the autosampler at 4°C, and analyzed within 48 h of reconstitution in buffer.