Summary of Study ST000259

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000210. The data can be accessed directly via it's Project DOI: 10.21228/M87W2S This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST000259
Study TitleMetabolic contribution of pSymA and pSymB megaplasmid/chromid for multipartite Sinorhizobium meliloti cultured in rich LBmc medium
Study Typemegaplasmid deletion
Study SummaryWe wished to evaluate the contribution of pSymA and pSymB towards the utilization of various metabolites in a nutritionally complex environment and to examine how S. meliloti influences its surrounding environment.
Institute
McMaster University
DepartmentDepartment of Biology
Last NameFinan
First NameTurlough
AddressDepartment of Biology, McMaster University, Hamilton, Canada L8S4K1
Emailfinan@mcmaster.ca
Phone(+1)905-525-9140 ext 22932
Submit Date2015-09-17
Num Groups14
Total Subjects82
Raw Data AvailableYes
Raw Data File Type(s)d
Analysis Type DetailLC-MS
Release Date2015-10-26
Release Version1
Turlough Finan Turlough Finan
https://dx.doi.org/10.21228/M87W2S
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Sample Preparation:

Sampleprep ID:SP000287
Sampleprep Summary:-
Sampleprep Protocol Filename:Fei,_F.,_Bowdish,_D._M._E.,_&_McCarry,_B._E._(2014)._Analytical_and_Bioanalytical_Chemistry,_406,_3723–3733.
Extraction Method:dilute 10 fold in 1:1 MeOH/EtOH
Extract Storage:-80ºC freezer
Sample Spiking:L-methionine-d3, L-tryptophan-d5 as standards for recovery determination ; L-phenylalanine-d8, diphenylalanine and glycine-phenylalanine as internal standards for peak area normalization
Cell Type:Gram negative bacterium
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