Summary of Study ST000261
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000212. The data can be accessed directly via it's Project DOI: 10.21228/M80C7B This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST000261 |
| Study Title | 1H NMR metabolomics study of spleen from C57BL/6 mice exposed to gamma radiation |
| Study Type | Tissue Extracts Comparison |
| Study Summary | Tissue extracts from exposure to gamma radiation mouse spleen and controls were compared via NMR based metabolomic analysis |
| Institute | Pacific Northwest National Laboratory |
| Department | Fundamental & Computational Sciences |
| Last Name | Hu |
| First Name | Jianzhi |
| Address | -- |
| jianzhi.hu@pnnl.gov | |
| Phone | -- |
| Submit Date | 2015-10-26 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | fid |
| Uploaded File Size | 24M |
| Analysis Type Detail | NMR |
| Release Date | 2015-10-26 |
| Release Version | 1 |
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Sample Preparation:
| Sampleprep ID: | SP000289 |
| Sampleprep Summary: | Cold Methanol/H2O/Chloroform Extraction |
| Sampleprep Protocol Comments: | Extraction protocol: Step 1: Weight each intact frozen tissue and add 0.25 ml methanol and 0.053 ml deionized water for each sample, after homogenizing in ice bath using the Tissue Tearor (Model 985-370, BioSpec Products, Inc.), then following by vortexing the mixture and then adding 0.125 ml chloroform, vortexing again. Step 2: 0.125 ml chloroform and 0.125 ml deionized water were added to the sample and then vortexed again, following by transferring different layers into glass vials separately with syringes after the mixture being left on ice for 15 min and centrifuged at 6,000 rpm for 16 min at 4 °C. Finally, the MeOH/H2O layer solvents (hydrophilic metabolites) were removed by employing the lyophilizer. And the extracts were stored at ?80 °C freezer before performing NMR measurements. |
