Summary of Study ST000402

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000314. The data can be accessed directly via it's Project DOI: 10.21228/M8V59S This work is supported by NIH grant, U2C- DK119886.


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Study IDST000402
Study TitleImpact of glucose on the central metabolome of C. minutissima
Study SummaryAxenic Chlorella minutissima (UTEX 2341) was grown under mixotrophic and autotrophic conditions to compare metabolome differences. The purpose of this study was to understand how glucose impacted the central metabolome of C. minutissima.
University of California, Davis
DepartmentGenome and Biomedical Sciences Facility
LaboratoryWCMC Metabolomics Core
Last NameFiehn
First NameOliver
Address1315 Genome and Biomedical Sciences Facility, 451 Health Sciences Drive, Davis, CA 95616
Phone(530) 754-8258
Submit Date2016-05-16
Raw Data AvailableYes
Raw Data File Type(s)peg
Analysis Type DetailGC-MS
Release Date2016-06-18
Release Version1
Oliver Fiehn Oliver Fiehn application/zip

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Sample Preparation:

Sampleprep ID:SP000430
Sampleprep Summary:Quenching 1. Add 5mL of quenching solvent to each 15mL tube, and let sit for at least 30 minutes in ice bath to get to temperature. 2. Add 1mL of cell culture (OD600=1.5) to tube, cap, and gently invert several times. 3. Centrifuge 1,000xg for 5 minutes. 4. Remove supernatant, and store supernatant and cell pellet in -80C until further use. Extraction 1. Add 0.5mL of extraction solvent to tube, gently pipet to remove all cells, transfer cells to 2mL eppendorf tube. Repeat for a total of 1mL extraction solvent + cells in 2mL eppendorf tube. 2. Add 2 small stainless steel grinding beads to eppendorf tube 3. Use the GenoGrinder to grind for 3 minutes at 1,250 rpm. 4. Centrifuge at 14,000xg for 5 minutes. 5. Transfer supernatant to a fresh 2mL eppendorf tube. 6. Add 1mL of extraction solvent to tube containing cell pellet + beads, and repeat steps 3 and 4. 7. Collect supernatant, and combine with supernatant collected in step 5. Total volume of extracted sample will be approximately 2mL. 8. Dry down 50uL of extracted sample in 1.5mL eppendorf tube for GC-TOF analysis. 9. Store backups in -20 or -80C. -20°C bath 5mL quenching solvent 1mL cells+broth
Sampleprep Protocol Filename:SOP_Extraction_of_Yeast_Cells.pdf