Summary of Study ST000420

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000329. The data can be accessed directly via it's Project DOI: 10.21228/M8R88H This work is supported by NIH grant, U2C- DK119886.

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Study ID	ST000420
Study Title	NIST SRM Smoker non-Smoker Urine Comparison
Study Summary	This study is a test of the untargeted metabolomics approach to studying samples from exposure studies. The samples are NIST standard SRM 3672 for pooled urine of smokers and SRM 3673 for pooled urine of non-smokers. A broad spectrum GCMS approach was used in this study.
Institute	RTI International
Department	Discovery Sciences
Last Name	Burgess
First Name	Jason
Address	3040 Cornwallis Rd, RTP, NC, 27609, USA
Email	jpb@rti.org
Phone	919-541-6700
Submit Date	2016-06-10
Study Comments	CHEAR ring trial profiiency testing
Chear Study	Yes
Analysis Type Detail	GC-MS
Release Date	2024-12-31
Release Version	1

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Sample Preparation:

Sampleprep ID:	SP000448
Sampleprep Summary:	Aliquots of 200 µL human urine were mixed with 20uL of urease suspension placed on Thermomixer for 1 hour at 37°C on 1400rpm. Then added 1.7mL of methanol, vortexed for 5 minutes, centrifuged for 10 minutes on 14,000 rpm at 4°C. 1.5 mL of the supernatant was transferred to new individual 2 mL Eppendorf tubes and dried completely under nitrogen flow. Placed on vacuum centrifuge for 30 min to ensure complete dryness. Formation of methoximes by adding 10µL of 40mg/mL MeOx in pyridine to each sample. Placed onto Thermomixer for 90 min at 30⁰C. Then FAME retention index markers and MSTFA added to each sample for derivatization step. Samples returned to Thermomixer for 45 min at 70⁰C.

Sampleprep ID:

SP000448

Sampleprep Summary:

Aliquots of 200 µL human urine were mixed with 20uL of urease suspension placed on Thermomixer for 1 hour at 37°C on 1400rpm. Then added 1.7mL of methanol, vortexed for 5 minutes, centrifuged for 10 minutes on 14,000 rpm at 4°C. 1.5 mL of the supernatant was transferred to new individual 2 mL Eppendorf tubes and dried completely under nitrogen flow. Placed on vacuum centrifuge for 30 min to ensure complete dryness. Formation of methoximes by adding 10µL of 40mg/mL MeOx in pyridine to each sample. Placed onto Thermomixer for 90 min at 30⁰C. Then FAME retention index markers and MSTFA added to each sample for derivatization step. Samples returned to Thermomixer for 45 min at 70⁰C.