Summary of Study ST000438

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000338. The data can be accessed directly via it's Project DOI: 10.21228/M8X01N This work is supported by NIH grant, U2C- DK119886.

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Study ID	ST000438
Study Title	Characterizing commonalities and differences between the breast and prostate cancer metabotypes in African-American cohorts (part I)
Study Type	Identify Breast Cancer Progression and Prostate Cancer correlative Metabolite Markers
Study Summary	Thus, we aimed to 1) understand mechanisms related to the onset and progression of BCa, 2) identify precursors and targets for prevention and therapy for each stage, grade and subtype which may contribute to the disparate impact of BCa in African American women, and 3) Identify common and different BCa metabolite markers versus PCa markers.
Institute	University of North Carolina
Department	NIH Eastern Regional Comprehensive Metabolomics Resource Core (RTI RCMRC)
Laboratory	Sumner Lab
Last Name	Sumner
First Name	Susan
Address	Eastern Regional Comprehensive Metabolomics Resource Core, UNC Nutrition Research Institute, 500 Laureate Way, Kannapolis, NC, 28081
Email	susan_sumner @unc.edu
Phone	704-250-5066
Submit Date	2016-08-02
Num Groups	2
Total Subjects	48
Num Females	48
Raw Data Available	Yes
Analysis Type Detail	NMR
Release Date	2016-09-23
Release Version	1

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Sample Preparation:

Sampleprep ID:	SP000466
Sampleprep Summary:	Aliquots of each de-identified sample were shipped to the NIH RTI-RCMRC on dry ice and immediately stored at -80 °C after being logged in for metabolomics analysis. A total of 48 study samples were weighed on dry ice to confirm weights and approximately 50 mg of the tissue was transferred to labeled MagNa Lyser bead tubes on ice and ice cold 50:50 acetonitrile:water was added, and samples were homogenized with two 30sec pulses at 3000rpm. Tubes were centrifuged at 16,000 rcf for 10 minutes at room temperature and supernatants were transferred to 1.5mL pre-labeled LoBind Eppendorf tubes. Aliquots of 500uL were then transferred into labeled 2.0mL LoBind Eppendorf tubes. Analytical quality control (QC) whole study pool samples were generated by transferring an additional 125µL aliquot of each study sample into a 10mL cyrovial and vortexed. To generate Total Pooled QC samples 500uL was transferred to 5 labeled 2.0mL LoBind Eppendorf tubes. All samples were lyophilized to complete dryness overnight, then reconstituted with 700uL of NMR Master Mix solution containing Chenomx ISTD: DSS-d6 and Phosphate Buffer at 7.4 pH. The tubes were vortexed for 4 min on a multi-tube vortexer and centrifuged at 16,000 rcf for 5 min. A 600uL aliquot of supernatants were transferred into a pre-labeled 5mm 4 NMR tubes for data acquisition on a 700 MHz spectrometer.

Sampleprep ID:

SP000466

Sampleprep Summary:

Aliquots of each de-identified sample were shipped to the NIH RTI-RCMRC on dry ice and immediately stored at -80 °C after being logged in for metabolomics analysis. A total of 48 study samples were weighed on dry ice to confirm weights and approximately 50 mg of the tissue was transferred to labeled MagNa Lyser bead tubes on ice and ice cold 50:50 acetonitrile:water was added, and samples were homogenized with two 30sec pulses at 3000rpm. Tubes were centrifuged at 16,000 rcf for 10 minutes at room temperature and supernatants were transferred to 1.5mL pre-labeled LoBind Eppendorf tubes. Aliquots of 500uL were then transferred into labeled 2.0mL LoBind Eppendorf tubes. Analytical quality control (QC) whole study pool samples were generated by transferring an additional 125µL aliquot of each study sample into a 10mL cyrovial and vortexed. To generate Total Pooled QC samples 500uL was transferred to 5 labeled 2.0mL LoBind Eppendorf tubes. All samples were lyophilized to complete dryness overnight, then reconstituted with 700uL of NMR Master Mix solution containing Chenomx ISTD: DSS-d6 and Phosphate Buffer at 7.4 pH. The tubes were vortexed for 4 min on a multi-tube vortexer and centrifuged at 16,000 rcf for 5 min. A 600uL aliquot of supernatants were transferred into a pre-labeled 5mm 4 NMR tubes for data acquisition on a 700 MHz spectrometer.