Summary of Study ST000441
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000340. The data can be accessed directly via it's Project DOI: 10.21228/M85G6J This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST000441 |
Study Title | Metabolomic Profiling of the Malaria Box Reveals Antimalarial Target Pathways |
Study Summary | Here we interrogated the in vitro metabolic effects of 189 drugs (including 169 of the drug-like compounds from the Malaria Box) using ultra-high performance liquid chromatography mass-spectrometry (UHPLC-MS). The resulting metabolic fingerprints provide information on the parasite biochemical pathways affected by pharmacologic intervention and offer a critical blueprint for selecting and advancing lead compounds as next-generation antimalarial drugs. Our results reveal several major classes of metabolic disruption, which allow us to predict the mode-of-action (MoA) for many of the Malaria Box compounds. |
Institute | Pennsylvania State University |
Last Name | Llinás |
First Name | Manuel |
Address | W126 Millenium Science Complex, University Park, PA 16802 |
manuel@psu.edu | |
Phone | (814) 867-3527 |
Submit Date | 2016-07-11 |
Publications | doi: 10.1128/AAC.01224-16 |
Raw Data File Type(s) | raw(Thermo) |
Analysis Type Detail | LC-MS |
Release Date | 2016-12-22 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP000469 |
Sampleprep Summary: | 90% Methanol/10% water at 50:1 solvent/cell pellet followed by vortexing and clarification via centrifugation |
Processing Method: | Lysis |
Processing Storage Conditions: | 4 C |
Extract Cleanup: | Clarification via centrifugation followed by nitrogen drying |
Extract Storage: | -80 C |
Sample Resuspension: | 6X pellet volume of HPLC H2O |
Sample Spiking: | Internal Standard 13C4, 15N1-Aspartate used for sample preparation normalizer |