Summary of study ST000450

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000348. The data can be accessed directly via it's Project DOI: 10.21228/M82K58 This work is supported by NIH grant, U2C- DK119886.

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Study IDST000450
Study TitleMetabolic features of chronic fatigue syndrome
Study TypePlasma metabolomic profiling
Study SummaryThis targeted metabolomic analysis was performed on plasma samples from 39 normal controls (n=18 men and 21 women) and 45 subjects ((n = 22 men and 23 women) who met diagnostic criteria for ME/CFS by Institute of Medicine, Canadian, and Fukuda criteria.
Institute
University of California, San Diego
DepartmentThe Mitochondrial and Metabolic Disease Center
Last NameNaviaux
First NameRobert
Address214 Dikinson Street, CTF-C102, San Diego, CA, 92103
Emailmaviaux@ucsd.edu
Phone619-993-2904
Submit Date2016-08-11
Num Groups2 groups for men (control and CFS) and 2 groups for women (control and CFS)
Total Subjects84
Analysis Type DetailLC-MS
Release Date2016-12-22
Release Version1
Robert Naviaux Robert Naviaux
https://dx.doi.org/10.21228/M82K58
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Sample Preparation:

Sampleprep ID:SP000478
Sampleprep Summary:None
Extraction Method:Typically, 90 μl of plasma was thawed on ice and mixed with 5 μl of isotope standards and incubated for 10 min at 20°C to permit small molecules and vitamins in the internal standards to associate with plasma binding proteins. Macromolecules (protein, DNA, RNA, glycans, etc.) were precipitated by extraction with 4 volumes (400 μl) of cold (-20°C), acetonitrile:methanol (50:50) and incubated on crushed ice for 10 min, then removed by centrifugation at 16,000g x 10 min at 4°C. The supernatants containing the extracted metabolites and internal standards were transferred to labeled cryotubes and stored at -80°C for LCMS/MS analysis.
Extract Storage:-80°C
Sample Derivatization:No
Sample Spiking:25-35 commercial stable isotope internal standards, and 57 stable isotope internal standards that were custom-synthesized in E. coli and S. cerevisiae by metabolic labeling with 13C-glucose and 13C bicarbonate
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