Summary of study ST000450

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000348. The data can be accessed directly via it's Project DOI: 10.21228/M82K58 This work is supported by NIH grant, U2C- DK119886.


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Study IDST000450
Study TitleMetabolic features of chronic fatigue syndrome
Study TypePlasma metabolomic profiling
Study SummaryThis targeted metabolomic analysis was performed on plasma samples from 39 normal controls (n=18 men and 21 women) and 45 subjects ((n = 22 men and 23 women) who met diagnostic criteria for ME/CFS by Institute of Medicine, Canadian, and Fukuda criteria.
University of California, San Diego
DepartmentThe Mitochondrial and Metabolic Disease Center
Last NameNaviaux
First NameRobert
Address214 Dikinson Street, CTF-C102, San Diego, CA, 92103
Submit Date2016-08-11
Num Groups2 groups for men (control and CFS) and 2 groups for women (control and CFS)
Total Subjects84
Analysis Type DetailLC-MS
Release Date2016-12-22
Release Version1
Robert Naviaux Robert Naviaux application/zip

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Sample Preparation:

Sampleprep ID:SP000478
Sampleprep Summary:None
Extraction Method:Typically, 90 μl of plasma was thawed on ice and mixed with 5 μl of isotope standards and incubated for 10 min at 20°C to permit small molecules and vitamins in the internal standards to associate with plasma binding proteins. Macromolecules (protein, DNA, RNA, glycans, etc.) were precipitated by extraction with 4 volumes (400 μl) of cold (-20°C), acetonitrile:methanol (50:50) and incubated on crushed ice for 10 min, then removed by centrifugation at 16,000g x 10 min at 4°C. The supernatants containing the extracted metabolites and internal standards were transferred to labeled cryotubes and stored at -80°C for LCMS/MS analysis.
Extract Storage:-80°C
Sample Derivatization:No
Sample Spiking:25-35 commercial stable isotope internal standards, and 57 stable isotope internal standards that were custom-synthesized in E. coli and S. cerevisiae by metabolic labeling with 13C-glucose and 13C bicarbonate