Summary of Study ST000478

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000364. The data can be accessed directly via it's Project DOI: 10.21228/M80K61 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

Study ID	ST000478
Study Title	NMR based Metabolomics Analysis of Liver from C57BL/6 Mouse Exposed to Ionizing Radiation
Study Summary	Tissue extracts from ionizing readiation exposed mouse liver and controls were compared via NMR based metabolomic analysis
Institute	Pacific Northwest National Laboratory
Department	Fundamental & Computational Sciences
Last Name	Hu
First Name	Jianzhi
Address	Pacific Northwest National Laboratory, Richland, WA 99352
Email	Jianzhi.Hu@pnnl.gov
Phone	+1 (509) 371-6544
Submit Date	2016-08-11
Raw Data Available	Yes
Raw Data File Type(s)	fid
Analysis Type Detail	NMR
Release Date	2017-07-10
Release Version	1

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Sample Preparation:

Sampleprep ID:	SP000506
Sampleprep Summary:	Step 1: After randomization of the samples, every preweighted intact frozen liver tissue was homogenized by a Tissue Tearsor (Model 985-370, BioSpec Product, Inc.) in ice-cold after adding 4 ml MeOH and 0.85 ml deionized H2O per gram of liver tissue, followed by vortexing the mixture and then 2 ml chloroform per gram of tissue was added and vortexed again. This process took 6 minutes and kept exactly the same for each sample. Step 2: 2 ml chloroform and 2 ml deionized H2O per gram of tissue were added in the mixture then vortexed again, followed by transferring the different layers into glass vials separately with syringes after the mixture (in ice bath) centrifuged. Finally, the solvents of hydrophilic metabolites were removed by employing lyophilizer and then stored at -80oC freezer before NMR spectral measurements.

Sampleprep ID:

SP000506

Sampleprep Summary:

Step 1: After randomization of the samples, every preweighted intact frozen liver tissue was homogenized by a Tissue Tearsor (Model 985-370, BioSpec Product, Inc.) in ice-cold after adding 4 ml MeOH and 0.85 ml deionized H2O per gram of liver tissue, followed by vortexing the mixture and then 2 ml chloroform per gram of tissue was added and vortexed again. This process took 6 minutes and kept exactly the same for each sample. Step 2: 2 ml chloroform and 2 ml deionized H2O per gram of tissue were added in the mixture then vortexed again, followed by transferring the different layers into glass vials separately with syringes after the mixture (in ice bath) centrifuged. Finally, the solvents of hydrophilic metabolites were removed by employing lyophilizer and then stored at -80oC freezer before NMR spectral measurements.