Summary of Study ST000501

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000378. The data can be accessed directly via it's Project DOI: 10.21228/M8659W This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

Study ID	ST000501
Study Title	NMR based Metabolomics Analysis of Mouse Exposed to Gamma Radiation
Study Type	Biofluids Extracts Comparison
Study Summary	Biofluid extracts from gamma readiation exposed mouse plasma and controls were compared via NMR based metabolomic analysis
Institute	Pacific Northwest National Laboratory
Last Name	Hu
First Name	Jianzhi
Address	3335 Innovation Blvd, Richland, Washington, 99354, USA
Email	Jianzhi.Hu@pnnl.gov
Phone	(509)371-6544
Submit Date	2016-11-02
Raw Data Available	Yes
Raw Data File Type(s)	fid
Chear Study	Yes
Analysis Type Detail	NMR
Release Date	2024-12-31
Release Version	1

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Sample Preparation:

Sampleprep ID:	SP000530
Sampleprep Summary:	Step 1: After randomization of the samples, every preweighted intact frozen plasma was added into MeOH and then vortex 2 mins. After centrifuge at 7200rpm for 5 minutes at 4C, the liqiuds layer in translted into a glass vial. Add 4 ml MeOH and 0.85 ml deionized H2O per gram of liver tissue, followed by vortexing the mixture and then 2 ml chloroform per gram of tissue was added and vortexed again. This process took 6 minutes and kept exactly the same for each sample. Step 2: 2 ml chloroform and 2 ml deionized H2O per gram of tissue were added in the mixture then vortexed again, followed by transferring the different layers into glass vials separately with syringes after the mixture (in ice bath) centrifuged. Finally, the solvents of hydrophilic metabolites were removed by employing lyophilizer and then stored at -80oC freezer before NMR spectral measurements.

Sampleprep ID:

SP000530

Sampleprep Summary:

Step 1: After randomization of the samples, every preweighted intact frozen plasma was added into MeOH and then vortex 2 mins. After centrifuge at 7200rpm for 5 minutes at 4C, the liqiuds layer in translted into a glass vial. Add 4 ml MeOH and 0.85 ml deionized H2O per gram of liver tissue, followed by vortexing the mixture and then 2 ml chloroform per gram of tissue was added and vortexed again. This process took 6 minutes and kept exactly the same for each sample. Step 2: 2 ml chloroform and 2 ml deionized H2O per gram of tissue were added in the mixture then vortexed again, followed by transferring the different layers into glass vials separately with syringes after the mixture (in ice bath) centrifuged. Finally, the solvents of hydrophilic metabolites were removed by employing lyophilizer and then stored at -80oC freezer before NMR spectral measurements.