Summary of Study ST000532

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000391. The data can be accessed directly via it's Project DOI: 10.21228/M8HG71 This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

Study ID	ST000532
Study Title	CHEAR Plasma Reference Material Plasma GC-MS
Study Type	Metabolomics
Study Summary	CHEAR Reference Material Plasma was provided by Emory University. The material was prepared and analyzed using the GC-TOF MS workflow of the Eastern Regional Metabolomics Resource Core (protocols available in metabolomics workbench). Seven samples were injected of the sample reference material prepared in replicate.
Institute	RTI International
Department	Discovery-Sciences-Technology (DST)
Laboratory	RTI CHEAR Analytical Hub - Untargeted Analysis Resource Core
Last Name	Fennell
First Name	Timothy
Address	3040 E Cornwallis Road, Research Triangle Park, NC 27709
Email	fennell@rti.org
Phone	919-485-2781
Submit Date	2016-12-30
Raw Data Available	Yes
Raw Data File Type(s)	cdf
Chear Study	Yes
Analysis Type Detail	GC-MS
Release Date	2024-12-31
Release Version	1

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Sample Preparation:

Sampleprep ID:	SP000561
Sampleprep Summary:	liquots of 30µL of plasma were mixed with 1000µL of (3:3:2) ACN:IPA:H2O by vortex mixer then centrifuged. Supernatant recovered. Archived 450µL of supernatant. Continued prep of remaining 450µL supernatant. Evaporated to dryness w/speed vac. Samples resuspended in 450µL of 1:1 ACN:H2O. Evaporated to dryness w/speed vac. Formation of methoximes by adding 10µL of 40mg/mL MeOx in pyridine to each sample. Placed onto Thermomixer for 90 min at 30⁰C. Then FAME retention index markers and MSTFA added to each sample for derivatization step. Samples returned to Thermomixer for 45 min at 70⁰C.

Sampleprep ID:

SP000561

Sampleprep Summary:

liquots of 30µL of plasma were mixed with 1000µL of (3:3:2) ACN:IPA:H2O by vortex mixer then centrifuged. Supernatant recovered. Archived 450µL of supernatant. Continued prep of remaining 450µL supernatant. Evaporated to dryness w/speed vac. Samples resuspended in 450µL of 1:1 ACN:H2O. Evaporated to dryness w/speed vac. Formation of methoximes by adding 10µL of 40mg/mL MeOx in pyridine to each sample. Placed onto Thermomixer for 90 min at 30⁰C. Then FAME retention index markers and MSTFA added to each sample for derivatization step. Samples returned to Thermomixer for 45 min at 70⁰C.