Summary of Study ST000553

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000405. The data can be accessed directly via it's Project DOI: 10.21228/M8Q01S This work is supported by NIH grant, U2C- DK119886.

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Study ID	ST000553
Study Title	CHEAR UT Urine and Plasma Pool Characterization
Study Summary	Analyze CHEAR UT urine and plasma pool in six replicates
Institute	Mount Sinai
Last Name	Andra
First Name	Syam
Address	Mount Sinai
Email	syam.andra@mssm.edu
Phone	212-241-7369
Submit Date	2017-02-14
Chear Study	Yes
Analysis Type Detail	LC-MS
Release Date	2024-12-31
Release Version	1

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Sample Preparation:

Sampleprep ID:	SP000582
Sampleprep Summary:	Sample preparation Plasma: Plasma samples were stored at -80°C until the day of the analysis. Metabolite extraction was performed by adding 300μL of cold LC-MS grade methanol (containing ISTDs) to 100μL of plasma. Samples were centrifuged to pellet the precipitate (13,000g, 15 min, 4°C). An aliquot of 60μL of the supernatants was transferred to 2 separate LC vials for Reversed-phase chromatography and positive and negative ESI mode detection. Extracts were evaporated to dryness using a speed vac system. The extracts were reconstituted using 50μL of methanol on the day of analysis. In case of the HILIC analysis (positive and negative ESI detection), the extracts were reconstituted using 50μL acetonitrile: water in 8:2 ratio. Urine: Urine samples were stored at -80°C until the day of the analysis. Metabolite extraction was performed by adding 180μL of Acetonitrile (containing ISTDs) to 20μL of urine. Samples were centrifuged to pellet the precipitate (13,000g, 15 min, 4°C). An aliquot of 40μL of the supernatants was transferred to 4 separate LC vials (RP and HILIC mode, positive and negative ESI analysis).

Sampleprep ID:

SP000582

Sampleprep Summary:

Sample preparation Plasma: Plasma samples were stored at -80°C until the day of the analysis. Metabolite extraction was performed by adding 300μL of cold LC-MS grade methanol (containing ISTDs) to 100μL of plasma. Samples were centrifuged to pellet the precipitate (13,000g, 15 min, 4°C). An aliquot of 60μL of the supernatants was transferred to 2 separate LC vials for Reversed-phase chromatography and positive and negative ESI mode detection. Extracts were evaporated to dryness using a speed vac system. The extracts were reconstituted using 50μL of methanol on the day of analysis. In case of the HILIC analysis (positive and negative ESI detection), the extracts were reconstituted using 50μL acetonitrile: water in 8:2 ratio. Urine: Urine samples were stored at -80°C until the day of the analysis. Metabolite extraction was performed by adding 180μL of Acetonitrile (containing ISTDs) to 20μL of urine. Samples were centrifuged to pellet the precipitate (13,000g, 15 min, 4°C). An aliquot of 40μL of the supernatants was transferred to 4 separate LC vials (RP and HILIC mode, positive and negative ESI analysis).