Summary of Study ST000569

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000417. The data can be accessed directly via it's Project DOI: 10.21228/M8531V This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

Study ID	ST000569
Study Title	Effect of minimal and complex media on the metabolite profiles
Study Summary	Media dependent intracellular metabolite changes of microorganisms
Institute	Graduate school of Korea University
Last Name	Kim
First Name	Jungyeon
Address	145, Anam-ro, Seongbuk-gu, Seoul, Seoul, 02841, Korea, South
Email	kim131812@korea.ac.kr
Phone	821082248015
Submit Date	2017-03-07
Study Comments	Each class means,Intracellular metabolites of E.coli cultivated in M9 Intracellular metabolites of E.coli cultivated in LB Intracellular metabolites of S.cerevisiae cultivated in YNB Intracellular metabolites of S.cerevisiae cultivated in YPD.
Raw Data Available	Yes
Raw Data File Type(s)	peg
Analysis Type Detail	GC-MS
Release Date	2017-07-10
Release Version	1

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Sample Preparation:

Sampleprep ID:	SP000598
Sampleprep Summary:	metabolite samples were derivatized in two steps. First, the samples were incubated with 5 µl of 40 mg/ml methoxyamine hydrochloride in pyridine (Sigma-Aldrich, St. Louis, MO) at 30 °C for 90 min, and then, with 45 µl of N-methyl-N-trimethylsilyl-trifluoroacetamide (Fluka, Buchs, Switzerland) at 37 °C for 30 min. A mixture of fatty acid methyl esters including methyl forms of C8, C9, C10, C12, C14, C16, C18, C20, C22, C24, C26, C28, and C30 was added to the derivatized sample as the retention index markers.

Sampleprep ID:

SP000598

Sampleprep Summary:

metabolite samples were derivatized in two steps. First, the samples were incubated with 5 µl of 40 mg/ml methoxyamine hydrochloride in pyridine (Sigma-Aldrich, St. Louis, MO) at 30 °C for 90 min, and then, with 45 µl of N-methyl-N-trimethylsilyl-trifluoroacetamide (Fluka, Buchs, Switzerland) at 37 °C for 30 min. A mixture of fatty acid methyl esters including methyl forms of C8, C9, C10, C12, C14, C16, C18, C20, C22, C24, C26, C28, and C30 was added to the derivatized sample as the retention index markers.