Summary of Study ST000890

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000619. The data can be accessed directly via it's Project DOI: 10.21228/M8NT1F This work is supported by NIH grant, U2C- DK119886.

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Study ID	ST000890
Study Title	Identification of RXR Ligands
Study Type	Idenfication of Ligands by HPLC-MS
Study Summary	Free fatty acids in mouse plasma were identified and quantified by LC-MS. Through differential feeding and PHZ (phnylhydrazine) dosing, coupled with mass spectrometry, we identified the long chain fatty acid C24:5 as a natural RXRA ligand, which was dynamically increased in concentration in response to hematopoietic stress. Collectively, these data demonstrate that natural RXRA ligands are present and are dynamically regulated in vivo in mouse hematopoietic cells.
Institute	Washington University in St. Louis
Department	Diabetic Cardiovascular Disease Center, School of Medicine
Laboratory	Metabolomics Core
Last Name	Fujiwara
First Name	Hideji
Address	660 South Euclid Ave, St. Louis MO 63110
Email	hfujiwar@wustl.edu
Phone	314-747-0494
Submit Date	2017-09-22
Study Comments	Units of measurement:peak area ratio: analyte peak area/peak area of internal standard
Analysis Type Detail	LC-MS
Release Date	2017-10-22
Release Version	1

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Sample Preparation:

Sampleprep ID:	SP000934
Sampleprep Summary:	For free fatty acids analysis, 100 μL of plasma extraction sample or 400 μL pull-down samples was used for DMAPA (dimethylaminopropylamine) derivatization for improving MS sensitivity of free fatty acids. Prior to the derivatization, 50 ng of AA-d8 (deuterated arachidonic acid –d8) as the internal standard was added to each sample. The solvents in the sample were dried under a stream of nitrogen. To derivatize the sample, 50 μL of 50 mM EDC (N-ethyl-N-(3-dimethylaminopropyl)-carbodiimide hydrochloride) and 50 mM DMAPA and 50 mM DMAP (4-dimethylaminopyridine) were added to the dried fatty acid samples and heated at 50 ◦C for 30 minutes. The samples were dried under nitrogen and then dissolved in 1 mL of ethanol : water for MS.

Sampleprep ID:

SP000934

Sampleprep Summary:

For free fatty acids analysis, 100 μL of plasma extraction sample or 400 μL pull-down samples was used for DMAPA (dimethylaminopropylamine) derivatization for improving MS sensitivity of free fatty acids. Prior to the derivatization, 50 ng of AA-d8 (deuterated arachidonic acid –d8) as the internal standard was added to each sample. The solvents in the sample were dried under a stream of nitrogen. To derivatize the sample, 50 μL of 50 mM EDC (N-ethyl-N-(3-dimethylaminopropyl)-carbodiimide hydrochloride) and 50 mM DMAPA and 50 mM DMAP (4-dimethylaminopyridine) were added to the dried fatty acid samples and heated at 50 ◦C for 30 minutes. The samples were dried under nitrogen and then dissolved in 1 mL of ethanol : water for MS.