Summary of Study ST000967
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000660. The data can be accessed directly via it's Project DOI: 10.21228/M8C39R This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST000967 |
| Study Title | Metabolomics Involved in Early-Life Single Pulse Antibiotic Exposures - Serum (part II) |
| Study Type | Timecourse treatment Vs control |
| Study Summary | The mice serum samples were extracted and analyzed using broad spectrum GCMS for the identification of compounds distinguishing the groups. |
| Institute | University of North Carolina |
| Department | NIH Eastern Regional Comprehensive Metabolomics Resource Core (RTI RCMRC) |
| Laboratory | UNC ERCMRC GCMS Core |
| Last Name | Sumner |
| First Name | Susan |
| Address | 500 Laureate Way, Kannapolis, NC 28081 |
| susan_sumner@unc.edu | |
| Phone | 704-250-5066 |
| Submit Date | 2018-04-20 |
| Num Groups | 10 |
| Total Subjects | 86 |
| Num Males | 30 |
| Num Females | 56 |
| Study Comments | groups include FRDA samples, Control samples, FRDA pools, Control pools, and Total pools |
| Raw Data Available | Yes |
| Raw Data File Type(s) | cdf |
| Analysis Type Detail | GC-MS |
| Release Date | 2018-08-02 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
| Sampleprep ID: | SP001013 |
| Sampleprep Summary: | Extraction, Solvent Removal, Resuspension, Solvent Removal, Two-step Derivatization and Addition of FAME Markers |
| Processing Method: | Aliquots of 30 µL serum were mixed with 1000 µL of degassed 3:3:2 Acetonitrile:Isopropanol:Water solution in a 2 mL snap cap tube. Samples were vortexed, shaken for 5 minutes then centrifuged at 4⁰C for 2 minutes at 14000rcf. The supernatant was recovered and split into two fractions. Fraction 1 (450 µL) was archived and fraction 2 (450 µL) was subjected to complete sample processing. Processing continued as follows. Samples were completely dried by vacuum centrifuge and reconstituted with 450 µL 1:1 acetonitrile:water solution. Samples were centrifuged for 2 minutes at 14000rcf and transferred to new snap cap tubes followed by complete evaporation at room temperature by vacuum centrifuge. |
| Processing Storage Conditions: | On ice |
| Extraction Method: | (step 1) 3:3:2 Acetonitrile:Isopropanol:H2O, (step 2) 1:1 Acetonitrile:H2O |
| Extract Storage: | -80℃ |
| Sample Resuspension: | Samples resuspended in 200 µL of 1:1 ACN:H2O. Evaporated to dryness w/speed vac. |
| Sample Derivatization: | Formation of methoximes by adding 10µL of 40mg/mL MeOx in pyridine to each sample. Placed onto Thermomixer for 90 min at 30⁰C. Then FAME retention index markers and MSTFA added to each sample for derivatization step. Samples returned to Thermomixer for 45 min at 70⁰C. |
| Sample Spiking: | Fatty acid methyl esters (FAME) |
