Summary of Study ST001051

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000704. The data can be accessed directly via it's Project DOI: 10.21228/M8P96G This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Study ID	ST001051
Study Title	High Salt Diet versus Low Salt Diet in rats
Study Summary	Two groups of rats were fed either a high salt diet or a low salt diet. This study aims to look at salt intake in correlation to altering other metabolites and the onset of hypertension
Institute	University of Toledo
Last Name	Chakraborty
First Name	Saroj
Address	3000 Arlington Ave. Toledo, Ohio 43614-2598
Email	Saroj.Chakraborty@rockets.utoledo.edu
Phone	419.383.5174
Submit Date	2018-09-12
Raw Data File Type(s)	cdf
Analysis Type Detail	GC-MS
Release Date	2018-10-10
Release Version	1

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Sample Preparation:

Sampleprep ID:	SP001100
Sampleprep Summary:	Samples were vortexed for 3 seconds to homogenize. Added 30 µL of each sample to a 1.5 mL eppendorf tube. Added 1 mL of extraction solvent (3:3:2 ACN:IPA:Water). Vortexed for 10 seconds. Shake for 6 minutes at 4°C. Centrifuged for 2 minutes at 14000 rcf. Aliquoted 2 x 475 µL portions of the supernatant into new tubes. Save one aliquote at -20°C for backup and dry the other in a centrivap to complete dryness. After the sample is dry, add 500 µL of 50:50 ACN:IPA. Centifuged for 2 minutes at 14000 rcf. Aliquoted 475 µL of supernatant to a new tube. Evaporate to complete dryness in centrivap. Submit for derivitzation.

Sampleprep ID:

SP001100

Sampleprep Summary:

Samples were vortexed for 3 seconds to homogenize. Added 30 µL of each sample to a 1.5 mL eppendorf tube. Added 1 mL of extraction solvent (3:3:2 ACN:IPA:Water). Vortexed for 10 seconds. Shake for 6 minutes at 4°C. Centrifuged for 2 minutes at 14000 rcf. Aliquoted 2 x 475 µL portions of the supernatant into new tubes. Save one aliquote at -20°C for backup and dry the other in a centrivap to complete dryness. After the sample is dry, add 500 µL of 50:50 ACN:IPA. Centifuged for 2 minutes at 14000 rcf. Aliquoted 475 µL of supernatant to a new tube. Evaporate to complete dryness in centrivap. Submit for derivitzation.