Summary of Study ST001073
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000718. The data can be accessed directly via it's Project DOI: 10.21228/M8VT2P This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
| Study ID | ST001073 |
| Study Title | Lipid profiling of Wnt3a-induced optic nerve regeneration |
| Study Type | untargeted lipid profiling |
| Study Summary | We analyzed lipid profiles of mouse retina and optic nerve for 2 time points - 7 and 15 days post-crush, and 3 conditions - intact control, optic nerve crush + vehicle (saline) intravitreal injection, optic nerve crush + 20 ng of Wnt3a injection. |
| Institute | University of Miami |
| Department | Ophthalmology, Bascom Palmer Eye Institute |
| Laboratory | Sanjoy K. Bhattacharya lab |
| Last Name | Bhattacharya |
| First Name | Sanjoy |
| Address | Bascom Palmer Eye Institute (McKnight Bldg.), 1638 NW 10th Avenue, Suite 707A, University of Miami, Miami, FL, 33136 |
| sbhattacharya@med.miami.edu | |
| Phone | 305-482-4103 |
| Submit Date | 2018-10-03 |
| Num Groups | 6 |
| Total Subjects | 12 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | raw(Thermo) |
| Analysis Type Detail | LC-MS |
| Release Date | 2018-12-11 |
| Release Version | 1 |
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Sample Preparation:
| Sampleprep ID: | SP001124 |
| Sampleprep Summary: | 4 mL of methanol (LC-MS grade) and 2 mL of chloroform (LC-MS grade) were added to each sample. After 2 min vigorous vortexing and 2 min sonication in ultrasonic bath, the samples were incubated at 48˚C overnight (in borosilicate glass vials, PTFE-lined caps). The following day, 2 mL of water (LC-MS grade) and 1 mL of chloroform were added, samples vigorously vortexed for 2 min and centrifuged at 3000 RCF, 4˚C for 15 min to obtain phase separation. Lower phases were collected and dried in centrifugal vacuum concentrator. Samples were stored at -20˚C until reconstituted in 150 µL of chloroform:methanol (1:1) prior to mass spectrometric analysis. Lipid profiling was performed in 2 batches: 7 day and 15 day period. For the 15 day period, quality control (QC) pooled sample was prepared and run 6 times throughout the batch. |
| Processing Method: | lipid extraction |
| Processing Storage Conditions: | Described in summary |
| Extraction Method: | chloroform, methanol, water phase separation |
| Extract Storage: | -80℃ |
| Sample Resuspension: | 150 µL of chloroform:methanol (1:1) |
| Sample Spiking: | no internal standards added |
