Summary of Study ST001109

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000740. The data can be accessed directly via it's Project DOI: 10.21228/M81D57 This work is supported by NIH grant, U2C- DK119886.

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Study ID	ST001109
Study Title	NMR Metabolomics of Newborn Heart Tissue Exposed to Excess Maternal Cortisol in Late Gestation - Maternal (part -IV)
Study Type	Comparison
Study Summary	Serum from mothers and fetuses exposed to excess maternal cortisol in late gestation and untreated was compared via NMR metabolomic analysis
Institute	University of Florida
Department	Biochemsitry & Molecular Biology
Last Name	Walejko
First Name	Jacquelyn
Address	R3-226 Academic Research Building, Department of Biochemistry and Molecular Biology, PO Box 100245, Gainesville, FL 32610-0245
Email	jwalejko@uga.edu
Phone	NA
Submit Date	2018-12-04
Num Groups	2
Total Subjects	36
Raw Data Available	Yes
Raw Data File Type(s)	fid
Analysis Type Detail	NMR
Release Date	2019-03-06
Release Version	1

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Sample Preparation:

Sampleprep ID:	SP001161
Sampleprep Summary:	Saline buffer was created by weighing 9 g of NaCl into a 1 L volumetric flask. Next, 100 mL of D2O was added and filled up to 1 L with water and shaken until dissolved. Samples were thawed on ice and centrifuged at 3,000 g for 20 min at 4 °C. Two hundred mL of serum was mixed with 400 mL of Saline buffer and centrifuged for 10 min at 3000 g at 4 °C. For each sample, 500 mL of the resulting supernatant was transferred into a 5 mm SampleJet NMR tube (Bruker Biospin, Billerica, MA, USA).

Sampleprep ID:

SP001161

Sampleprep Summary:

Saline buffer was created by weighing 9 g of NaCl into a 1 L volumetric flask. Next, 100 mL of D2O was added and filled up to 1 L with water and shaken until dissolved. Samples were thawed on ice and centrifuged at 3,000 g for 20 min at 4 °C. Two hundred mL of serum was mixed with 400 mL of Saline buffer and centrifuged for 10 min at 3000 g at 4 °C. For each sample, 500 mL of the resulting supernatant was transferred into a 5 mm SampleJet NMR tube (Bruker Biospin, Billerica, MA, USA).