Summary of study ST001131

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000756. The data can be accessed directly via it's Project DOI: 10.21228/M8ZH5N This work is supported by NIH grant, U2C- DK119886.

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Study IDST001131
Study TitlePresentation of different serum metabolites in trauma patients versus healthy volunteers.
Study SummaryThis study seeks to identify critical metabolic differences between patients who have undergone physical trauma and patients who have not experienced physical trauma.
Institute
Texas Tech University
DepartmentUniversity Health Sciences Center
Last NameMudaliar
First NameNithya
Address3601 4th St, Lubbock, TX 79430
Emailnithya.s.mudaliar@ttuhsc.edu
Phone8067436794
Submit Date2019-01-28
Num Groups2
Total Subjects12
Raw Data AvailableYes
Raw Data File Type(s).cdf
Analysis Type DetailLC-MS
Release Date2019-03-06
Release Version1
Nithya Mudaliar Nithya Mudaliar
https://dx.doi.org/10.21228/M8ZH5N
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Sample Preparation:

Sampleprep ID:SP001200
Sampleprep Summary:All extraction procedures are performed over crushed ice. Samples were defrosted over crushed ice for 30 minutes or until defrosted. 30 µL of serum was aliquoted from the original sample into a 1.5 mL Eppendorf tube. 1 mL of 3:3:2 ACN:IPA:Water was added to each sample. Samples were vortexed for 10 seconds. Samples were shaken for 6 minutes at 4°C using an orbital mixing plate. Samples were centrifuged for 2 minutes at 14,000 rcf. 2 aliquots of 475 µL were removed from the supernatant and placed into new 1.5 mL Eppendorf tubes. 1 sample is frozen at -20°C and saved as a backup. The other sample is dried to completeness using a Labconco Centrivap.
Sample Derivatization:To the dried down samples, 10 µL of a prepared methoxyamine solution was added to each sample. Samples were shaken on an Orbital Mixing Chilling/Heating Plate at 30°C for 1.5 hours. 91 µL of a N-methyl-N-(trimethylsilyl)-trifluoroacetamide (MSTFA) and fatty-acid methyl-esters (FAMES) solution was added to each sample. Samples were shaken on an Orbital Mixing Chilling/Heating Plate at 37°C for 30 minutes. Contents were transferred to individual glass vials with micro-inserts and capped immediately. Submitted to GC-MS analysis.
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