Summary of Study ST001133

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000758. The data can be accessed directly via it's Project DOI: 10.21228/M8Q10R This work is supported by NIH grant, U2C- DK119886.


This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

Perform statistical analysis  |  Show all samples  |  Show named metabolites  |  Download named metabolite data  |  Perform analysis on untargeted data  
Download mwTab file (text)   |  Download mwTab file(JSON)   |  Download data files
Study IDST001133
Study TitleDownregulation of CENPF epigenetically remodels prostate cancer cells and alters cellular metabolism
Study SummaryThis study aimed to determine the metabolic profile of CENPF-knockout (CENPFKO) PC cells and identify differentially expressed metabolites (DEMs) that can be used as diagnostic markers.
Cedars-Sinai Medical Center
Last NameKim
First NameJayoung
Address8700 Beverly Blvd., Davis 5071 Los Angeles, CA 90048, USA
Submit Date2019-01-14
Analysis Type DetailGC-MS
Release Date2020-01-06
Release Version1
Jayoung Kim Jayoung Kim application/zip

Select appropriate tab below to view additional metadata details:

Sample Preparation:

Sampleprep ID:SP001202
Sampleprep Summary:Samples were dissolved in 1 ml -20'C mixture of acetonitrile, isopropanol, and water (3:3:2 v/v) at a pH of 7. The solution was then vortexed at 4'C for 5 min. Samples were centrifuged for 2 min at 14,000 rcf and 500 ul were aliquoted. The aliquots were then evaporated in a Labconco Centrivap Cold Trap to complete dryness. The methoximation step was performed using a 10 ul solution of 40 mg/ml O-methylhydroxylamine hydrochloride (CAS: [593-56-6]; Formula: CH5NO.HCl) and shaken for 90 min at 30C. Then, 90 ul of N-methyl-N-trimethylsilyltrifluoroacetamide (MSTFA) was added and the solution was shaken for 30 min at 37'C. After, a 1 ul mixture of fatty acid methyl ester (FAME) retention time markers were added. The mixture was transferred to amber crimp autosampler vials.