Summary of Study ST001134

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000759. The data can be accessed directly via it's Project DOI: 10.21228/M8K67W This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study IDST001134
Study TitleGC-TOF-MS, Brassica nigra / MeJA + herbivory
Study SummaryGC-TOF-MS, Brassica nigra / MeJA + herbivory
Institute
Umeå Plant Science Centre
DepartmentPlant Physiology
Last NamePapazian
First NameStefano
AddressUmeå University
Emailstefano.papazian@umu.se
Phone0046723160854
Submit Date2019-02-06
Num Groups4
Total Subjects55
Study CommentsMeasurements are chromatographic peak area normalized by internal standard, arbritrary units.
Raw Data AvailableYes
Raw Data File Type(s)cdf
Analysis Type DetailGC-MS
Release Date2019-08-06
Release Version1
Stefano Papazian Stefano Papazian
https://dx.doi.org/10.21228/M8K67W
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Sample Preparation:

Sampleprep ID:SP001203
Sampleprep Summary:Each sample (10-12 mg) was extracted in 1 ml of cold chloroform:methanol:H2O (20:60:20 v/v), including 7.5 ng/µl of the isotope-labelled internal standard, SA-D4. Extracts were agitated with a 3 mm tungsten carbide bead for 3 min and centrifuged at 20,800 × g for 10 min at 4°C. The supernatant (200 μl) was dried and processed for GC/MS analyses
Processing Storage Conditions:On ice
Extract Storage:-80℃
Sample Derivatization:Dry extracts were derivatized with methoxyamine and MSTFA
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