Summary of Study ST001141

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000762. The data can be accessed directly via it's Project DOI: 10.21228/M86103 This work is supported by NIH grant, U2C- DK119886.

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Study ID	ST001141
Study Title	Evaluation of metabolome sample preparation and extraction methodologies for oleaginous filamentous fungi Mortierella alpina
Study Type	method optimization
Study Summary	In this study, based on the method of fast filtration, we evaluated the three metabolomics analysis protocols commonly used for microbial metabolomics analysis in M. alpina and systematically optimised the metabolite extraction solvent.
Institute	Jiangnan University
Department	School of Food Science and Technology
Laboratory	State Key Laboratory of Food Science and Technology
Last Name	Hengqian
First Name	Lu
Address	1800 Lihu Ave, Wuxi, Jiangsu 214122, P.R. China.
Email	hengqianlu@163.com
Phone	+86 15006176136
Submit Date	2019-02-24
Analysis Type Detail	GC-MS
Release Date	2019-09-23
Release Version	1

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Sample Preparation:

Sampleprep ID:	SP001213
Sampleprep Summary:	The obtained supernatants were vacuum dried at 30°C prior to derivatisation. The extraction solution served as a negative control to subtract from the background spectrum. The dried extract was derivatised. Briefly, the vacuum dried samples were resuspended in 100 μL of MeOX (10 mg/mL) in pyridine and incubated at 37°C for 90 min in a block heater. Then, 40 μL of MSTFA+1%TMCS was pipetted into each sample and incubated at 37°C for another 30 min. The resulting solution from each sample was collected and transferred into gas chromatography (GC) vials for analysis.

Sampleprep ID:

SP001213

Sampleprep Summary:

The obtained supernatants were vacuum dried at 30°C prior to derivatisation. The extraction solution served as a negative control to subtract from the background spectrum. The dried extract was derivatised. Briefly, the vacuum dried samples were resuspended in 100 μL of MeOX (10 mg/mL) in pyridine and incubated at 37°C for 90 min in a block heater. Then, 40 μL of MSTFA+1%TMCS was pipetted into each sample and incubated at 37°C for another 30 min. The resulting solution from each sample was collected and transferred into gas chromatography (GC) vials for analysis.