Summary of Study ST001166
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000727. The data can be accessed directly via it's Project DOI: 10.21228/M8Q38G This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
Study ID | ST001166 |
Study Title | Physiological and metabolic response of pteropods to ocean acidification (part IV) |
Study Summary | The objective of the study was to examine the physiological and metabolic response of pteropods to ocean acidification treatment. Four treatments were used:high pH, high DO (dissolved oxygen); high pH, low DO; low pH, high DO; low pH, low DO |
Institute | NOAA NWFSC |
Department | CB Division |
Last Name | Nichols |
First Name | Krista |
Address | 1315 East-West Highway Silver Spring, MD 20910 |
krista.nichols@noaa.gov | |
Phone | 206-302-2470 |
Submit Date | 2019-04-05 |
Num Groups | 4 |
Total Subjects | 60 |
Analysis Type Detail | LC-MS |
Release Date | 2019-05-15 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP001239 |
Sampleprep Summary: | Crabs were removed from -80°C. 15 mg of muscle tissue was removed from each crab and placed in a 2 mL eppendorph tube. 2 x 3mm grinding beads were added to each sample. 225 µL of methanol with quality control standards were added to each sample. Samples were ground for 30 seconds at 1500 rpm using a GenoGrinder. 750 µL of methyl-tertbutyl ether (MTBE) was added to each sample. Samples were vortexed for 10 seconds and then shaked at 4°C for 6 minutes. 188 µL of LC-MS grade water was added to each sample. Samples were vortexed for 10 seconds and then centrifuged for 2 minutes at 14,000 rcf. The top, organic layer was split into two 350 µL aliquots, one submitted to lipidomics analysis and one saved for backup. The bottom layer was split into two 125 µL aliquots, one submitted to GC analysis and one saved as a backup. |