Summary of Study ST001170

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench,, where it has been assigned Project ID PR000782. The data can be accessed directly via it's Project DOI: 10.21228/M8M10T This work is supported by NIH grant, U2C- DK119886.


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Study IDST001170
Study TitleTimecourse on MCF-7 cells treated with different concentration of doxorubicin
Study SummaryMCF-7 cells treated with 10μM doxorubicin for 4h followed by subsequent withdrawl of the drug and cultured up to 48h.Doxurubicin-treated cells and control cells were collected at 0,12,24,36,and 48h. Meanwhile,MCF-7 cells continuously exposed to a low dosage of doxorubicin at 0.1μM for 96h. Doxorubicin-treated cells and control cells were collected at 0,24,48,72 and 96h.
China Pharmaceutical University
Last NameShao
First NameChang
AddressTongjiaxiang #24, Nanjing, Jiangsu, 210000, China
Submit Date2019-04-11
Raw Data AvailableYes
Raw Data File Type(s)qgd
Analysis Type DetailLC-MS
Release Date2020-12-01
Release Version1
Chang Shao Chang Shao application/zip

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Sample Preparation:

Sampleprep ID:SP001243
Sampleprep Summary:MCF-7 cells were harvested and permeabilized with cold (80% (v/v) methanol: water solution followed by a 20-min incubation at -80 ℃. A stock solution of 4-Chloro-DL-phenylalanine was pre-spiked into the methanol aqueous buffer to a final concentration of 1.5 μg/mL, which subsequently served as an internal standard (IS). The cell lysates were centrifuged at 14,000g for 5 min at 4 ℃, and the resultant supernatant were collected and evaporated to dryness. The samples were reconstituted in 100 μL of water (LC-MS grade) before analysis on an HPLC−MS/MS, and 20 μL was used for analysis. For each group of samples subjected to metabolomics analysis, five biological replicates were prepared.