Summary of Study ST001179

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000791. The data can be accessed directly via it's Project DOI: 10.21228/M8F97P This work is supported by NIH grant, U2C- DK119886.

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Study ID	ST001179
Study Title	Metabolomic analysis of skeletal muscle in young and aged mice
Study Summary	Sarcopenia is the age-induced, progressive loss of skeletal muscle mass and function, which results in poor muscle performance. To better understand changes in skeletal muscles during sarcopenia, we performed a metabolomic analysis of skeletal muscle in young (8-week-old) and aged (28-month-old) mice using CE-TOFMS. Our data shows that the metabolites including glucose and polyamine metabolism were decreased in aged mice compared with young mice. In addition, neurotransmitter levels were higher in aged mice.
Institute	Kyoto Prefectural University
Last Name	Kamei
First Name	Yasutomi
Address	1-5 Hangi-cho, Shimogamo, Sakyo-ku Kyoto, Kyoto, Kyoto, 606-8522, Japan
Email	ran960116@yahoo.co.jp
Phone	+81-75-703-5661
Submit Date	2019-05-06
Analysis Type Detail	CE-MS
Release Date	2019-07-17
Release Version	1

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Sample Preparation:

Sampleprep ID:	SP001253
Sampleprep Summary:	Gastrocnemius muscles of 8-week-old male mice (N=5, young group) and 28-month-old male mice (N=5, aged group) were used for the metabolomic analysis (Human Metabolome Technologies Inc., Tsuruoka, Japan). Frozen muscle specimens were transferred into 500 μL of methanol containing 50 mM external standard. After homogenization by BMSM10N21 (BMS, Tokyo) at 1,500 rpm for 120 s performed 5 times, 500 μL of chloroform and 200 μL of ultrapure water were added to the homogenate. The solution was mixed well and centrifuged at 2,300 × g for 5 min at 4 °C. The resultant water phase was ultrafiltered using a Millipore Ultrafree-MC PLHCC HMT Centrifugal Filter Device, 5 kDa (Millipore, Billerica, MA). The filtrate was dried and then dissolved in 50 μL of ultrapure water. The samples obtained were then subjected to CE-TOFMS analysis using the Agilent CE-TOFMS system (Agilent Technologies, Santa Clara, CA) at 4 °C. The detected peaks were aligned according to their m/z values and normalized migration times. The peaks were mean-centered and scaled using their standard deviations on a per peak basis as a pretreatment.

Sampleprep ID:

SP001253

Sampleprep Summary:

Gastrocnemius muscles of 8-week-old male mice (N=5, young group) and 28-month-old male mice (N=5, aged group) were used for the metabolomic analysis (Human Metabolome Technologies Inc., Tsuruoka, Japan). Frozen muscle specimens were transferred into 500 μL of methanol containing 50 mM external standard. After homogenization by BMSM10N21 (BMS, Tokyo) at 1,500 rpm for 120 s performed 5 times, 500 μL of chloroform and 200 μL of ultrapure water were added to the homogenate. The solution was mixed well and centrifuged at 2,300 × g for 5 min at 4 °C. The resultant water phase was ultrafiltered using a Millipore Ultrafree-MC PLHCC HMT Centrifugal Filter Device, 5 kDa (Millipore, Billerica, MA). The filtrate was dried and then dissolved in 50 μL of ultrapure water. The samples obtained were then subjected to CE-TOFMS analysis using the Agilent CE-TOFMS system (Agilent Technologies, Santa Clara, CA) at 4 °C. The detected peaks were aligned according to their m/z values and normalized migration times. The peaks were mean-centered and scaled using their standard deviations on a per peak basis as a pretreatment.