Summary of Study ST001179

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000791. The data can be accessed directly via it's Project DOI: 10.21228/M8F97P This work is supported by NIH grant, U2C- DK119886.

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Study IDST001179
Study TitleMetabolomic analysis of skeletal muscle in young and aged mice
Study SummarySarcopenia is the age-induced, progressive loss of skeletal muscle mass and function, which results in poor muscle performance. To better understand changes in skeletal muscles during sarcopenia, we performed a metabolomic analysis of skeletal muscle in young (8-week-old) and aged (28-month-old) mice using CE-TOFMS. Our data shows that the metabolites including glucose and polyamine metabolism were decreased in aged mice compared with young mice. In addition, neurotransmitter levels were higher in aged mice.
Institute
Kyoto Prefectural University
Last NameKamei
First NameYasutomi
Address1-5 Hangi-cho, Shimogamo, Sakyo-ku Kyoto, Kyoto, Kyoto, 606-8522, Japan
Emailran960116@yahoo.co.jp
Phone+81-75-703-5661
Submit Date2019-05-06
Analysis Type DetailLC-MS
Release Date2019-07-17
Release Version1
Yasutomi Kamei Yasutomi Kamei
https://dx.doi.org/10.21228/M8F97P
ftp://www.metabolomicsworkbench.org/Studies/ application/zip

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Sample Preparation:

Sampleprep ID:SP001253
Sampleprep Summary:Gastrocnemius muscles of 8-week-old male mice (N=5, young group) and 28-month-old male mice (N=5, aged group) were used for the metabolomic analysis (Human Metabolome Technologies Inc., Tsuruoka, Japan). Frozen muscle specimens were transferred into 500 μL of methanol containing 50 mM external standard. After homogenization by BMSM10N21 (BMS, Tokyo) at 1,500 rpm for 120 s performed 5 times, 500 μL of chloroform and 200 μL of ultrapure water were added to the homogenate. The solution was mixed well and centrifuged at 2,300 × g for 5 min at 4 °C. The resultant water phase was ultrafiltered using a Millipore Ultrafree-MC PLHCC HMT Centrifugal Filter Device, 5 kDa (Millipore, Billerica, MA). The filtrate was dried and then dissolved in 50 μL of ultrapure water. The samples obtained were then subjected to CE-TOFMS analysis using the Agilent CE-TOFMS system (Agilent Technologies, Santa Clara, CA) at 4 °C. The detected peaks were aligned according to their m/z values and normalized migration times. The peaks were mean-centered and scaled using their standard deviations on a per peak basis as a pretreatment.
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