Summary of Study ST001185
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000796. The data can be accessed directly via it's Project DOI: 10.21228/M8SM3C This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
Study ID | ST001185 |
Study Title | Genetic and metabolic characterization of bioengineered human fatty liver tissue with modified SIRT1 expression |
Study Summary | Lipidomics and metabolomics was performed three types of tissue samples to compare human normal liver tissue against human NASH liver and the bioengineered human iPS-derived fatty liver tissue-iKD-SIRT1. The purpose of this study was to show that the global lipidomics profile of iPS-derived fatty liver tissue-iKD-SIRT1 was similar to that of patients with NASH |
Institute | University of Pittsburgh |
Department | Department of Pathology |
Last Name | Soto-Gutierrez |
First Name | Alejandro |
Address | 200 Lothrop Street, 423 Biomedical Science Tower, Pittsburgh, PA 15261, USA |
als208@pitt.edu | |
Phone | +14126480064 |
Submit Date | 2019-05-20 |
Num Groups | 3 |
Raw Data Available | Yes |
Analysis Type Detail | LC-MS |
Release Date | 2020-01-06 |
Release Version | 1 |
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Sample Preparation:
Sampleprep ID: | SP001259 |
Sampleprep Summary: | Human normal liver, human NASH liver and human iPS-derived fatty liver tissueiKD-SIRT1 samples were homogenized using a FastPrep system (MP Bio) with Matrix D ceramic beads in 80% MeOH at a ratio of 15 μL/mg tissue. The homogenate was spiked with isotopically labelled standards, taurine-1,1,2,2-d4 (final concentration 100 μM, Cambridge Isotopes MA) and 10 μL of a 50 μg/mL fatty acid internal standard mix. Chloroform (600 μL) was then added to the homogenate supernatant and the sample was vortexed and centrifuged at 1,500 x g for 5 min. The aqueous phase was taken for polar metabolite analysis and the organic phase was split for targeted free fatty acid analysis and untargeted lipidomics. Polar samples were cleared by centrifugation at 16,000 x g and the supernatant dried under N2. Samples were resuspended in 50 μL of 1.5 mM ammonium fluoride (aq) and 10 μL was injected for separation and analysis. The organic phase was dried under N2 and reconstituted in 100 μL of chloroform:methanol (2:1) and 5 μL was injected for untargeted lipidomics analysis. |