Summary of Study ST001212
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000823. The data can be accessed directly via it's Project DOI: 10.21228/M89D5Q This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001212 |
| Study Title | Fish-oil supplementation in pregnancy, child metabolomics and asthma risk |
| Study Summary | We investigated potential metabolic mechanisms using untargeted liquid chromatography-mass spectrometry-based metabolomics on 577 plasma samples collected at age 6 months in the offspring of mothers participating in the n-3 LCPUFA randomized controlled trial. First, associations between the n-3 LCPUFA supplementation groups and child metabolite levels were investigated using univariate regression models and data-driven partial least square discriminant analyses (PLS-DA). Second, we analyzed the association between the n-3 LCPUFA metabolomic profile and asthma development using Cox-regression. Third, we conducted mediation analyses to investigate whether the protective effect of n-3 LCPUFA on asthma was mediated via the metabolome |
| Institute | University of Copenhagen |
| Department | Copenhagen Prospective Studies on Asthma in Childhood, Herlev and Gentofte Hospital |
| Last Name | Rago |
| First Name | Daniela |
| Address | Ledreborg Alle 34, DK-2820 Gentofte; Denmark |
| daniela.rago@dbac.dk | |
| Phone | (+45) 39777360 |
| Submit Date | 2019-07-10 |
| Analysis Type Detail | LC-MS |
| Release Date | 2019-09-23 |
| Release Version | 1 |
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Sample Preparation:
| Sampleprep ID: | SP001306 |
| Sampleprep Summary: | The sample preparation was done using the automated system MicroLab STAR® system from Hamilton Company. Prior to extraction, the samples were fortified with recovery standards for quality control (QC) purposes. Methanol was used to extract the metabolites and precipitate the proteins during vigorous shaking for 2 min (Glen Mills GenoGrinder 2000) followed by centrifugation. The resulting extract was divided into four aliquots to be subsequently analyzed by LC-MS/MS comprising of four platforms. Samples were placed on a TurboVap® (Zymark) to remove the organic solvent and stored overnight under nitrogen before preparation for LC-MS/MS analysis. |
| Processing Storage Conditions: | -80℃ |
