Summary of Study ST001231

This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000824. The data can be accessed directly via it's Project DOI: 10.21228/M85Q5K This work is supported by NIH grant, U2C- DK119886.

See: https://www.metabolomicsworkbench.org/about/howtocite.php

This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.

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Study ID	ST001231
Study Title	Plasma untargeted metabolomics study of pulmonary tuberculosis
Study Summary	In this study, differentially abundant plasma metabolites were screened by using the ultra-high performance liquid chromatography coupled with Q Exactive mass spectrometry in pulmonary tuberculosis(TB) patients and normal controls(NC) or patients with other pulmonary diseases such as, community-acquired pneumonia(CAP) and lung cancer(LC).
Institute	Zhengjiang University
Department	School of Medicine
Laboratory	Institute of Cell Biology
Last Name	Li
First Name	Ji-Cheng
Address	866 Yuhangtang Road, West Lake District, Hangzhou, Zhejiang Province, 310058, PR China
Email	lijichen@zju.edu.cn
Phone	+86-571-88208088
Submit Date	2019-08-05
Raw Data File Type(s)	raw(Thermo)
Analysis Type Detail	LC-MS
Release Date	2020-12-01
Release Version	1

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Sample Preparation:

Sampleprep ID:	SP001307
Sampleprep Summary:	The frozen heparin anti-coagulated plasma samples were thawed on ice and 100 μl of each sample was transferred to a 1.5ml sterile micro-centrifugen tube. After the addition of 300 μl of methanol (containing internal standard L-2-chlorophenylalanine 1 μg/ml), each sample was vortexed for 30 s, sonicated for 10 min in ice-water bath, and incubated for 1 h at -20°C to precipitate proteins. Then each sample was centrifuged at 12000 rpm for 15 min at 4°C. The resulting supernatants were transferred to LC-MS vials and stored at -80°C until the UPLC-QE-MS analysis. Each quality control sample was also prepared by mixing an equal aliquot of the supernatants from each sample. The treatment methods were the same as above.

Sampleprep ID:

SP001307

Sampleprep Summary:

The frozen heparin anti-coagulated plasma samples were thawed on ice and 100 μl of each sample was transferred to a 1.5ml sterile micro-centrifugen tube. After the addition of 300 μl of methanol (containing internal standard L-2-chlorophenylalanine 1 μg/ml), each sample was vortexed for 30 s, sonicated for 10 min in ice-water bath, and incubated for 1 h at -20°C to precipitate proteins. Then each sample was centrifuged at 12000 rpm for 15 min at 4°C. The resulting supernatants were transferred to LC-MS vials and stored at -80°C until the UPLC-QE-MS analysis. Each quality control sample was also prepared by mixing an equal aliquot of the supernatants from each sample. The treatment methods were the same as above.