Summary of Study ST001234
This data is available at the NIH Common Fund's National Metabolomics Data Repository (NMDR) website, the Metabolomics Workbench, https://www.metabolomicsworkbench.org, where it has been assigned Project ID PR000827. The data can be accessed directly via it's Project DOI: 10.21228/M8SD6F This work is supported by NIH grant, U2C- DK119886.
See: https://www.metabolomicsworkbench.org/about/howtocite.php
This study contains a large results data set and is not available in the mwTab file. It is only available for download via FTP as data file(s) here.
| Study ID | ST001234 |
| Study Title | The effect of weaning stress, sex and temperament on fecal microbiota and metabolites in Brahman calves. |
| Study Summary | The objective of this study was to conduct a cross-sectional study to 1) investigate the effect of weaning on the fecal microbiota as well as serum metabolites in Bos indicus (Brahman) calves, and 2) compare the fecal microbiota as well as serum metabolites between males (bulls) and females (heifers) as well as between calm and temperamental animals at weaning (d0) and 4 days post weaning (d4). Equal numbers of animals were present in each category (5 calm female, 5 temperamental female, 5 calm male, and 5 temperamental male animals). |
| Institute | Texas A&M University |
| Department | Veterinary Pathobiology |
| Last Name | Lawhon |
| First Name | Sara |
| Address | MS4467 |
| slawhon@tamu.edu | |
| Phone | 979-218-7156 |
| Submit Date | 2019-08-06 |
| Raw Data Available | Yes |
| Raw Data File Type(s) | cdf |
| Analysis Type Detail | GC-MS |
| Release Date | 2024-08-06 |
| Release Version | 1 |
Select appropriate tab below to view additional metadata details:
Sample Preparation:
| Sampleprep ID: | SP001310 |
| Sampleprep Summary: | Blood serum was extracted following the protocols published in Fiehn et al. PLoS ONE (2010) e15234. A 30 ul aliquot of each sample was extracted with 1 ml of degassed acetonitrile : isopropanol : water (3:3:2, v/v/v) at -20C, centrifuged and decanted with subsequent evaporation of the solvent to complete dryness. A clean-up step with acetonitrile/water (1:1) was used to remove membrane lipids and triglycerides. The cleaned extract was aliquoted into two equal portions and the supernatant was dried again. Internal standards C08-C30 FAMEs were added and the samples were derivatized by methoxyamine hydrochloride in pyridine and subsequently by N-methyl-N-trimethylsilyltrifluoroacetamide for trimethylsilylation of acidic protons. |
| Processing Storage Conditions: | -20℃ |
| Extraction Method: | A 30 ul aliquot of each sample was extracted with 1 ml of degassed acetonitrile : isopropanol : water (3:3:2, v/v/v) at -20C, centrifuged and decanted with subsequent evaporation of the solvent to complete dryness. |
| Extract Cleanup: | A clean-up step with acetonitrile/water (1:1) was used to remove membrane lipids and triglycerides. |
| Extract Storage: | -20℃ |
| Sample Derivatization: | Samples were derivatized by methoxyamine hydrochloride in pyridine and subsequently by N-methyl-N-trimethylsilyltrifluoroacetamide for trimethylsilylation of acidic protons. |
| Sample Spiking: | Internal standards C08-C30 FAMEs were added. |
